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pubmed-article:16511132pubmed:abstractTextXanthomonas campestris pv. campestris (Xcc) is a Gram-negative yellow-pigmented bacterium and is the causative agent of black rot, one of the major worldwide diseases of cruciferous crops. It also synthesizes a variety of polyketide metabolites that lead to important antibiotics. XC5357 is a putative 12.2 kDa protein of unknown structure from Xcc that is likely to be essential for polyketide synthesis. It was overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. The crystals belong to the triclinic space group P1, with unit-cell parameters a = 43.7, b = 43.7, c = 46.5 A, alpha = 65.0, beta = 64.9, gamma = 73.4 degrees, and diffracted to a resolution of 1.85 A.lld:pubmed
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pubmed-article:16511132pubmed:authorpubmed-author:ChouChia-Chen...lld:pubmed
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pubmed-article:16511132pubmed:dateRevised2010-9-15lld:pubmed
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pubmed-article:16511132pubmed:articleTitleA putative polyketide-synthesis protein XC5357 from Xanthomonas campestris: heterologous expression, crystallization and preliminary X-ray analysis.lld:pubmed
pubmed-article:16511132pubmed:affiliationInstitute of Biochemistry, National Chung-Hsing University, Taichung 40227, Taiwan.lld:pubmed
pubmed-article:16511132pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:16511132pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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