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pubmed-article:1650909pubmed:abstractTextThe rnh gene of Escherichia coli encodes RNase H. rnh mutants display at least two phenotypes: (1) they require functional RecBCD enzyme for growth; thus rnh-339::cat recB270 (Ts) and rnh-339::cat recC271 (Ts) strains are temperature sensitive for growth; (2) rnh mutants permit replication that is independent of the chromosomal origin, presumably by failing to remove RNA-DNA hybrids from which extra-original replication can be primed. We report here that manifestation of these two phenotypes occurs at different levels of RNase H function; we have examined partially functional rnh mutants for their in vitro RNase H activity, their ability to rescue viability in recB or recC cells and their ability to permit growth of mutants incapable of using oriC [dnaA (Ts)].lld:pubmed
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pubmed-article:1650909pubmed:dateRevised2007-11-15lld:pubmed
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pubmed-article:1650909pubmed:articleTitleCorrelation of activity with phenotypes of Escherichia coli partial function mutants of rnh, the gene encoding RNase H.lld:pubmed
pubmed-article:1650909pubmed:affiliationLaboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.lld:pubmed
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