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pubmed-article:16497154pubmed:abstractTextTo support the study of kinin pharmacology in bovine models of cultured endothelial cells (ECs), the Bovine Genome Project was searched for a B1 receptor (B1R) gene ortholog (BDKRB1). A contig complementary to an intronless coding nucleotide sequence was found. The sequence was amplified from bovine EC DNA, further cloned into pcDNA3 and expressed in COS-1 cells. The bovine B1R sequence was confirmed and extended. A putative Zn2+-binding motif HEXXH is not present (replaced by HDAWP). The receptor binds [3H]Lys-des-Arg9-bradykinin in a saturable manner (K(d) 0.36 nM) and exhibits a pharmacological profile similar to that of human B(1)R.lld:pubmed
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pubmed-article:16497154pubmed:authorpubmed-author:MarceauFranço...lld:pubmed
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pubmed-article:16497154pubmed:articleTitleMolecular identification and pharmacological profile of the bovine kinin B1 receptor.lld:pubmed
pubmed-article:16497154pubmed:affiliationCentre de Recherche en Rhumatologie et Immunologie, Centre Hospitalier Universitaire de Québec, Québec City G1V 4G2, Canada.lld:pubmed
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