| pubmed-article:16495221 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16495221 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:16495221 | lifeskim:mentions | umls-concept:C0027950 | lld:lifeskim |
| pubmed-article:16495221 | lifeskim:mentions | umls-concept:C0079460 | lld:lifeskim |
| pubmed-article:16495221 | lifeskim:mentions | umls-concept:C0003695 | lld:lifeskim |
| pubmed-article:16495221 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:16495221 | lifeskim:mentions | umls-concept:C1948023 | lld:lifeskim |
| pubmed-article:16495221 | lifeskim:mentions | umls-concept:C1157321 | lld:lifeskim |
| pubmed-article:16495221 | pubmed:issue | 15 | lld:pubmed |
| pubmed-article:16495221 | pubmed:dateCreated | 2006-4-10 | lld:pubmed |
| pubmed-article:16495221 | pubmed:abstractText | Priming of human neutrophils with granulocyte-macrophage colony-stimulating factor (GM-CSF) followed by treatment with formyl-methionyl-leucyl-phenylalanine (fMLP) stimulates cells in a physiologically relevant manner with modest 5-lipoxygenase activation and formation of leukotrienes. However, pretreatment of neutrophils with thimerosal, an organomercury thiosalicylic acid derivative, led to a dramatic increase (>50-fold) in the production of leukotriene B(4) and 5-hydroxyeicosatetraenoic acid, significantly higher than that observed after stimulation with calcium ionophore A23187. Little or no effect was observed with thimerosal alone or in combination with either GM-CSF or fMLP. Elevation of [Ca(2+)](i) induced by thimerosal in neutrophils stimulated with GM-CSF/fMLP was similar but more sustained compared with samples where thimerosal was absent. However, [Ca(2+)](i) was significantly lower compared with calcium ionophore-treated cells, suggesting that a sustained calcium rise was necessary but not sufficient to explain the effects of this compound on the GM-CSF/fMLP-stimulated neutrophil. Thimerosal was found to directly inhibit neutrophil lysophospholipid:acyl-CoA acyltransferase activity at the doses that stimulate leukotriene production, and analysis of lysates from neutrophil preparations stimulated in the presence of thimerosal showed a marked increase in free arachidonic acid, supporting the inhibition of the reincorporation of this fatty acid into the membrane phospholipids as a mechanism of action for this compound. The dramatic increase in production of leukotrienes by neutrophils when a physiological stimulus such as GM-CSF/fMLP is employed in the presence of thimerosal suggests a critical regulatory role of arachidonate reacylation that limits leukotriene biosynthesis in concert with 5-lipoxygenase and cytosolic phospholipase A(2)alpha activation. | lld:pubmed |
| pubmed-article:16495221 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16495221 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:16495221 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:16495221 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16495221 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:16495221 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:16495221 | pubmed:author | pubmed-author:MurphyRobert... | lld:pubmed |
| pubmed-article:16495221 | pubmed:author | pubmed-author:FolcoGiancarl... | lld:pubmed |
| pubmed-article:16495221 | pubmed:author | pubmed-author:ZariniSimonaS | lld:pubmed |
| pubmed-article:16495221 | pubmed:author | pubmed-author:GijónMiguel... | lld:pubmed |
| pubmed-article:16495221 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16495221 | pubmed:day | 14 | lld:pubmed |
| pubmed-article:16495221 | pubmed:volume | 281 | lld:pubmed |
| pubmed-article:16495221 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16495221 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16495221 | pubmed:pagination | 10134-42 | lld:pubmed |
| pubmed-article:16495221 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
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| pubmed-article:16495221 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16495221 | pubmed:articleTitle | Effect of arachidonic acid reacylation on leukotriene biosynthesis in human neutrophils stimulated with granulocyte-macrophage colony-stimulating factor and formyl-methionyl-leucyl-phenylalanine. | lld:pubmed |
| pubmed-article:16495221 | pubmed:affiliation | Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, 12801 E. 17th Avenue, Aurora, CO 80045, USA. | lld:pubmed |
| pubmed-article:16495221 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16495221 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:16495221 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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