| pubmed-article:16489761 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16489761 | lifeskim:mentions | umls-concept:C0521009 | lld:lifeskim |
| pubmed-article:16489761 | lifeskim:mentions | umls-concept:C0026336 | lld:lifeskim |
| pubmed-article:16489761 | lifeskim:mentions | umls-concept:C0025255 | lld:lifeskim |
| pubmed-article:16489761 | lifeskim:mentions | umls-concept:C2709248 | lld:lifeskim |
| pubmed-article:16489761 | lifeskim:mentions | umls-concept:C0023810 | lld:lifeskim |
| pubmed-article:16489761 | lifeskim:mentions | umls-concept:C0377274 | lld:lifeskim |
| pubmed-article:16489761 | lifeskim:mentions | umls-concept:C0332256 | lld:lifeskim |
| pubmed-article:16489761 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:16489761 | pubmed:dateCreated | 2006-2-21 | lld:pubmed |
| pubmed-article:16489761 | pubmed:abstractText | We have reported that Gram-negative organisms decorated with rough lipopolysaccharide (LPS) are particularly susceptible to the direct antimicrobial actions of the pulmonary collectins, surfactant proteins A (SP-A) and D (SP-D). In this study, we examined the lipid and LPS components required for the permeabilizing effects of the collectins on model bacterial membranes. Liposomes composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), with or without rough Escherichia coli LPS (J5), smooth E. coli LPS (B5), or cholesterol, were loaded with self-quenching probes and exposed to native or oxidatively modified SP-A. Fluorescence that resulted from permeabilization of liposomes and diffusion of dyes was assessed by microscopy or fluorimetry. Human SP-A and melittin increased the permeability of J5 LPS/POPE liposomes, but not B5 LPS/POPE liposomes or control (POPE only) liposomes. At a human SP-A concentration of 100 microg/mL, the permeability of the J5 LPS/POPE membranes increased 4.4-fold (p < 0.02) compared to the control with no added SP-A. Rat SP-A and SP-D also permeabilized the J5-containing liposomes. Incorporation of cholesterol into J5 LPS/POPE liposomes at a POPE:cholesterol molar ratio of 1:0.15 blocked human SP-A or melittin-induced permeability (p < 0.05) compared to cholesterol-free liposomes. Exposure of human SP-A to surfactant lipid peroxidation blocked the permeabilizing activity of the protein. We conclude that SP-A permeabilizes phospholipid membranes in an LPS-dependent and rough LPS-specific manner, that the effect is neither SP-A- nor species-specific, and that oxidative damage to SP-A abolishes its membrane destabilizing properties. Incorporation of cholesterol into the membrane enhances resistance to permeabilization by SP-A, most likely by increasing the packing density and membrane rigidity. | lld:pubmed |
| pubmed-article:16489761 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16489761 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16489761 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16489761 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16489761 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:16489761 | pubmed:issn | 0006-2960 | lld:pubmed |
| pubmed-article:16489761 | pubmed:author | pubmed-author:McCormackFran... | lld:pubmed |
| pubmed-article:16489761 | pubmed:author | pubmed-author:WuHuixingH | lld:pubmed |
| pubmed-article:16489761 | pubmed:author | pubmed-author:KuzmenkoAlexa... | lld:pubmed |
| pubmed-article:16489761 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16489761 | pubmed:day | 28 | lld:pubmed |
| pubmed-article:16489761 | pubmed:volume | 45 | lld:pubmed |
| pubmed-article:16489761 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16489761 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16489761 | pubmed:pagination | 2679-85 | lld:pubmed |
| pubmed-article:16489761 | pubmed:dateRevised | 2011-8-25 | lld:pubmed |
| pubmed-article:16489761 | pubmed:meshHeading | pubmed-meshheading:16489761... | lld:pubmed |
| pubmed-article:16489761 | pubmed:meshHeading | pubmed-meshheading:16489761... | lld:pubmed |
| pubmed-article:16489761 | pubmed:meshHeading | pubmed-meshheading:16489761... | lld:pubmed |
| pubmed-article:16489761 | pubmed:meshHeading | pubmed-meshheading:16489761... | lld:pubmed |
| pubmed-article:16489761 | pubmed:meshHeading | pubmed-meshheading:16489761... | lld:pubmed |
| pubmed-article:16489761 | pubmed:meshHeading | pubmed-meshheading:16489761... | lld:pubmed |
| pubmed-article:16489761 | pubmed:meshHeading | pubmed-meshheading:16489761... | lld:pubmed |
| pubmed-article:16489761 | pubmed:meshHeading | pubmed-meshheading:16489761... | lld:pubmed |
| pubmed-article:16489761 | pubmed:meshHeading | pubmed-meshheading:16489761... | lld:pubmed |
| pubmed-article:16489761 | pubmed:meshHeading | pubmed-meshheading:16489761... | lld:pubmed |
| pubmed-article:16489761 | pubmed:meshHeading | pubmed-meshheading:16489761... | lld:pubmed |
| pubmed-article:16489761 | pubmed:meshHeading | pubmed-meshheading:16489761... | lld:pubmed |
| pubmed-article:16489761 | pubmed:meshHeading | pubmed-meshheading:16489761... | lld:pubmed |
| pubmed-article:16489761 | pubmed:meshHeading | pubmed-meshheading:16489761... | lld:pubmed |
| pubmed-article:16489761 | pubmed:meshHeading | pubmed-meshheading:16489761... | lld:pubmed |
| pubmed-article:16489761 | pubmed:meshHeading | pubmed-meshheading:16489761... | lld:pubmed |
| pubmed-article:16489761 | pubmed:meshHeading | pubmed-meshheading:16489761... | lld:pubmed |
| pubmed-article:16489761 | pubmed:meshHeading | pubmed-meshheading:16489761... | lld:pubmed |
| pubmed-article:16489761 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16489761 | pubmed:articleTitle | Pulmonary collectins selectively permeabilize model bacterial membranes containing rough lipopolysaccharide. | lld:pubmed |
| pubmed-article:16489761 | pubmed:affiliation | Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Cincinnati, 231 Albert Sabin Way, Cincinnati, Ohio 45267-0564, USA. | lld:pubmed |
| pubmed-article:16489761 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16489761 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:16489761 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
