| pubmed-article:16472807 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16472807 | lifeskim:mentions | umls-concept:C0035668 | lld:lifeskim |
| pubmed-article:16472807 | lifeskim:mentions | umls-concept:C0035687 | lld:lifeskim |
| pubmed-article:16472807 | lifeskim:mentions | umls-concept:C0080125 | lld:lifeskim |
| pubmed-article:16472807 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:16472807 | lifeskim:mentions | umls-concept:C0039679 | lld:lifeskim |
| pubmed-article:16472807 | lifeskim:mentions | umls-concept:C1709059 | lld:lifeskim |
| pubmed-article:16472807 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:16472807 | pubmed:dateCreated | 2006-2-28 | lld:pubmed |
| pubmed-article:16472807 | pubmed:abstractText | The internal guiding sequence (IGS) is normally located at the 5' end of trans-splicing ribozymes that are derived from the Tetrahymena group I intron, and is required for the recognition of substrate RNAs and for trans-splicing reactions. Here, we separated the Tetrahymena group I intron at the L2 loop to produce two fragments: the IGS-containing substrate, and the IGS-lacking ribozyme. We show here that two fragments can complex not through the IGS interaction but under the guidance of appended interacting nucleotides, and perform trans-splicing. The splicing reactions took place both in vitro and in mammalian cells, and the spliced mRNA product from the self-assembled ribozyme complex can be translated into functional proteins in vivo. The splicing efficiency was dependent on the length of appending nucleotides. | lld:pubmed |
| pubmed-article:16472807 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16472807 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16472807 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16472807 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16472807 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16472807 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16472807 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16472807 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16472807 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:16472807 | pubmed:issn | 0014-5793 | lld:pubmed |
| pubmed-article:16472807 | pubmed:author | pubmed-author:HasegawaSumit... | lld:pubmed |
| pubmed-article:16472807 | pubmed:author | pubmed-author:RaoJianghongJ | lld:pubmed |
| pubmed-article:16472807 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16472807 | pubmed:day | 6 | lld:pubmed |
| pubmed-article:16472807 | pubmed:volume | 580 | lld:pubmed |
| pubmed-article:16472807 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16472807 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16472807 | pubmed:pagination | 1592-6 | lld:pubmed |
| pubmed-article:16472807 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:16472807 | pubmed:meshHeading | pubmed-meshheading:16472807... | lld:pubmed |
| pubmed-article:16472807 | pubmed:meshHeading | pubmed-meshheading:16472807... | lld:pubmed |
| pubmed-article:16472807 | pubmed:meshHeading | pubmed-meshheading:16472807... | lld:pubmed |
| pubmed-article:16472807 | pubmed:meshHeading | pubmed-meshheading:16472807... | lld:pubmed |
| pubmed-article:16472807 | pubmed:meshHeading | pubmed-meshheading:16472807... | lld:pubmed |
| pubmed-article:16472807 | pubmed:meshHeading | pubmed-meshheading:16472807... | lld:pubmed |
| pubmed-article:16472807 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16472807 | pubmed:articleTitle | Modulating the splicing activity of Tetrahymena ribozyme via RNA self-assembly. | lld:pubmed |
| pubmed-article:16472807 | pubmed:affiliation | Biophysics Program, Department of Radiology, Stanford University School of Medicine, 1201 Welch Road, Stanford, CA 94305-5484, USA. | lld:pubmed |
| pubmed-article:16472807 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16472807 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:16472807 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
