| pubmed-article:16445580 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16445580 | lifeskim:mentions | umls-concept:C0021289 | lld:lifeskim |
| pubmed-article:16445580 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:16445580 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:16445580 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:16445580 | lifeskim:mentions | umls-concept:C0162638 | lld:lifeskim |
| pubmed-article:16445580 | lifeskim:mentions | umls-concept:C0033559 | lld:lifeskim |
| pubmed-article:16445580 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:16445580 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:16445580 | pubmed:dateCreated | 2006-1-31 | lld:pubmed |
| pubmed-article:16445580 | pubmed:abstractText | The aim of the present study was to investigate the effect of prostaglandin (PG) E1 on hypoxia/re-oxygenation (H/R) apoptosis and the expression of bcl-2 and bax in cultured neonatal rat cardiomyocytes. The H/R model was made using the first generation of cultured neonatal rat cardiomyocytes. Hypoxia/re-oxygenation apoptosis was studied by electron microscopy and agarose gel electrophoresis. The percentage of apoptotic cells was measured by terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labeling (TUNEL). The expression of bcl-2 and bax was detected by in situ hybridization and immunohistochemical staining. Most cells of the H/R group tested by electron microscopy showed cytoplasmic concentration, nuclear chromatin condensation and margination. Prostaglandin E1 (5, 15 and 45 microg/L) relieved the injury. The results of DNA electrophoresis in the H/R group showed a typical DNA ladder and the DNA ladder decreased gradually corresponding with increasing doses of PGE1. The TUNEL staining showed that the total number of apoptotic cells in the H/R group was much more than that in the PGE1 (45 microg/L) group. The results of in situ hybridization and immunohistochemical staining showed that the bcl-2 content in the H/R group was lower than that in the control group; bax content showed the reverse. Compared with the H/R group, bcl-2 content was significantly higher in the PGE1 (5, 15 and 45 microg/L) groups. However, bax content in the PGE1 (5, 15 and 45 microg/L) groups was significantly lower than that in the H/R group. 6. In conclusion, H/R injury can induce cardiomyocyte apoptosis. Prostaglandin E1 obviously has anti-apoptotic effects on cardiomyocytes and the mechanisms probably involve the inhibition of bax expression and increased expression of bcl-2. | lld:pubmed |
| pubmed-article:16445580 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16445580 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16445580 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16445580 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16445580 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16445580 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16445580 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16445580 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16445580 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16445580 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:16445580 | pubmed:issn | 0305-1870 | lld:pubmed |
| pubmed-article:16445580 | pubmed:author | pubmed-author:MaXiang-QinXQ | lld:pubmed |
| pubmed-article:16445580 | pubmed:author | pubmed-author:FuRun-FangRF | lld:pubmed |
| pubmed-article:16445580 | pubmed:author | pubmed-author:FengGuo-QingG... | lld:pubmed |
| pubmed-article:16445580 | pubmed:author | pubmed-author:WangZhen-JiZJ | lld:pubmed |
| pubmed-article:16445580 | pubmed:author | pubmed-author:MaShou-GuoSG | lld:pubmed |
| pubmed-article:16445580 | pubmed:author | pubmed-author:WengShi-AiSA | lld:pubmed |
| pubmed-article:16445580 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16445580 | pubmed:volume | 32 | lld:pubmed |
| pubmed-article:16445580 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16445580 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16445580 | pubmed:pagination | 1124-30 | lld:pubmed |
| pubmed-article:16445580 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:16445580 | pubmed:meshHeading | pubmed-meshheading:16445580... | lld:pubmed |
| pubmed-article:16445580 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:16445580 | pubmed:articleTitle | Hypoxia-reoxygenation-induced apoptosis in cultured neonatal rat cardiomyocyets and the protective effect of prostaglandin E. | lld:pubmed |
| pubmed-article:16445580 | pubmed:affiliation | Department of Pharmacology, College of Basic Sciences, Zhengzhou University, Zhengzhou, China. | lld:pubmed |
| pubmed-article:16445580 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16445580 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:24224 | entrezgene:pubmed | pubmed-article:16445580 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16445580 | lld:pubmed |
