| pubmed-article:16441426 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16441426 | lifeskim:mentions | umls-concept:C0039195 | lld:lifeskim |
| pubmed-article:16441426 | lifeskim:mentions | umls-concept:C1335376 | lld:lifeskim |
| pubmed-article:16441426 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:16441426 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:16441426 | pubmed:dateCreated | 2006-1-30 | lld:pubmed |
| pubmed-article:16441426 | pubmed:abstractText | Peptide-MHC tetramers have been engineered to allow accurate detection of antigen-specific cytotoxic C lymphocytes (CTL) by flow cytometry. Here, we propose a novel use for peptide-MHC tetramers in the specific and sensitive analysis of the cytotoxic function of antigen-specific CTL by blocking MHC-restricted antigen-specific cytotoxicity. We found that pretreatment of ovalbumin (OVA)-specific CD8(+) CTL (OT-1 CTL), derived from OT-1 T-cell receptor (TCR)-transgenic mice, with OVA(257-264) peptide-H-2K(b) tetramer caused a marked inhibition of the cytotoxicity against OVA-expressing EG-7 tumor cells. OVA(257-264) peptide-H-2K(b) tetramer did not block the cytotoxicity mediated by 2C mouse (H-2(b))-derived CD8(+) CTL, which recognize allo (H-2L(d)) antigens. Moreover, OT-I CTL activity was not inhibited by an irrelevant HBV(208-216) peptide-H-2K(b) tetramer. These results indicate that the blocking of CTL activity with peptide-MHC tetramer was caused by interference with the interaction between the TCR and H-2K(b)-OVA(257-264) peptide complex, but not with the CD8-MHC class I interaction. The blocking activity of OVA(257-264) peptide-H-2K(b) tetramer was reversible because OT-I CTL pretreated with the tetramer recovered their cytotoxicity after culturing with interleukin-2 for 24 h. The same results were also demonstrated in freshly isolated, in vivo-primed OT-1 CTL sorted by the tetramer. These results demonstrate that peptide-MHC tetramer is a useful tool for defining MHC-restricted antigen-specific CTL function. Moreover, our finding implies that the measurement of CTL activity immediately after tetramer-guided sorting is not a suitable method for evaluating the function of in vivo-induced tetramer-positive CTL. We believe that the tetramer-blocking assay presented here will be useful for functionally monitor the induction of MHC-restricted antigen-specific CTL during vaccination therapy against tumor and infectious diseases. | lld:pubmed |
| pubmed-article:16441426 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16441426 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16441426 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16441426 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16441426 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16441426 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16441426 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16441426 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16441426 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16441426 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16441426 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:16441426 | pubmed:issn | 1347-9032 | lld:pubmed |
| pubmed-article:16441426 | pubmed:author | pubmed-author:IkedaHiroakiH | lld:pubmed |
| pubmed-article:16441426 | pubmed:author | pubmed-author:NishimuraTaka... | lld:pubmed |
| pubmed-article:16441426 | pubmed:author | pubmed-author:YamazakiKoich... | lld:pubmed |
| pubmed-article:16441426 | pubmed:author | pubmed-author:NishimuraMasa... | lld:pubmed |
| pubmed-article:16441426 | pubmed:author | pubmed-author:Dosaka-AkitaH... | lld:pubmed |
| pubmed-article:16441426 | pubmed:author | pubmed-author:YokouchiHiros... | lld:pubmed |
| pubmed-article:16441426 | pubmed:author | pubmed-author:ChamotoKenjiK | lld:pubmed |
| pubmed-article:16441426 | pubmed:author | pubmed-author:WakitaDaikoD | lld:pubmed |
| pubmed-article:16441426 | pubmed:author | pubmed-author:NoguchiDaisuk... | lld:pubmed |
| pubmed-article:16441426 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16441426 | pubmed:volume | 97 | lld:pubmed |
| pubmed-article:16441426 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16441426 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16441426 | pubmed:pagination | 148-54 | lld:pubmed |
| pubmed-article:16441426 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:16441426 | pubmed:meshHeading | pubmed-meshheading:16441426... | lld:pubmed |
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| pubmed-article:16441426 | pubmed:meshHeading | pubmed-meshheading:16441426... | lld:pubmed |
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| pubmed-article:16441426 | pubmed:meshHeading | pubmed-meshheading:16441426... | lld:pubmed |
| pubmed-article:16441426 | pubmed:meshHeading | pubmed-meshheading:16441426... | lld:pubmed |
| pubmed-article:16441426 | pubmed:meshHeading | pubmed-meshheading:16441426... | lld:pubmed |
| pubmed-article:16441426 | pubmed:meshHeading | pubmed-meshheading:16441426... | lld:pubmed |
| pubmed-article:16441426 | pubmed:meshHeading | pubmed-meshheading:16441426... | lld:pubmed |
| pubmed-article:16441426 | pubmed:meshHeading | pubmed-meshheading:16441426... | lld:pubmed |
| pubmed-article:16441426 | pubmed:meshHeading | pubmed-meshheading:16441426... | lld:pubmed |
| pubmed-article:16441426 | pubmed:meshHeading | pubmed-meshheading:16441426... | lld:pubmed |
| pubmed-article:16441426 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16441426 | pubmed:articleTitle | Tetramer-blocking assay for defining antigen-specific cytotoxic T lymphocytes using peptide-MHC tetramer. | lld:pubmed |
| pubmed-article:16441426 | pubmed:affiliation | Division of Immunoregulation, Section of Disease Control, Institute for Genetic Medicine, Hokkaido University, Kita-ku, Sapporo 060-8638, Japan. | lld:pubmed |
| pubmed-article:16441426 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16441426 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:16441426 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
