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pubmed-article:16439107pubmed:dateCreated2006-8-25lld:pubmed
pubmed-article:16439107pubmed:abstractTextAn ultrasensitive piezoelectric immunosensor using an amplification path based on an insoluble biocatalyzed precipitation product has proposed for Schistosoma japonicum. A mercapto Schistosoma japonicum antigen was self-assembled onto the quartz crystal surface via an Au nanoparticle mediator monolayer to sense the Schistosoma japonicum antibody (SjAb). And the horseradish peroxidase labeled protein A conjugate which was bounded to the SjAb by a "sandwich" format was used as a biocatalyst for the oxidative precipitation of 4-chloro-1-naphthol by H(2)O(2) to yield the insoluble product benzo-4-chlorohexadienone, resulting in an amplified mass sensing of antigen-antibody interaction. The amount of the precipitate accumulated on the quartz crystal is controlled by the antibody concentration. The SjAb can be linearly determined in the range of 10-200 ngml(-1) and the detection limit reaches as low as 5 ngml(-1).lld:pubmed
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pubmed-article:16439107pubmed:monthAuglld:pubmed
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pubmed-article:16439107pubmed:authorpubmed-author:WuJianJlld:pubmed
pubmed-article:16439107pubmed:authorpubmed-author:WuZhaoyangZlld:pubmed
pubmed-article:16439107pubmed:authorpubmed-author:ShenGuoliGlld:pubmed
pubmed-article:16439107pubmed:authorpubmed-author:YuRuqinRlld:pubmed
pubmed-article:16439107pubmed:authorpubmed-author:WangShipingSlld:pubmed
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pubmed-article:16439107pubmed:pagination207-12lld:pubmed
pubmed-article:16439107pubmed:dateRevised2009-7-14lld:pubmed
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pubmed-article:16439107pubmed:year2006lld:pubmed
pubmed-article:16439107pubmed:articleTitleAn amplified mass piezoelectric immunosensor for Schistosoma japonicum.lld:pubmed
pubmed-article:16439107pubmed:affiliationState Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China. zuwu@hnu.cnlld:pubmed
pubmed-article:16439107pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:16439107pubmed:publicationTypeComparative Studylld:pubmed
pubmed-article:16439107pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
pubmed-article:16439107pubmed:publicationTypeValidation Studieslld:pubmed