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pubmed-article:16385549pubmed:abstractTextThe overlapping approach was developed recently to expand the adeno-associated viral (AAV) packaging capacity. In this approach, a gene is split into two partially overlapping fragments and separately packaged into an upstream and a downstream vector, respectively. Transgene expression is achieved in co-infected cells after homologous recombination. Despite the promising proof-of-principle results in the lung, the efficiency has been very disappointing in skeletal muscle. Here we examined two potential rate-limiting factors including AAV serotype and the transgene sequence.lld:pubmed
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pubmed-article:16385549pubmed:authorpubmed-author:DuanDongsheng...lld:pubmed
pubmed-article:16385549pubmed:authorpubmed-author:YueYongpingYlld:pubmed
pubmed-article:16385549pubmed:authorpubmed-author:GhoshArkasubh...lld:pubmed
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pubmed-article:16385549pubmed:dateRevised2011-4-20lld:pubmed
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pubmed-article:16385549pubmed:articleTitleViral serotype and the transgene sequence influence overlapping adeno-associated viral (AAV) vector-mediated gene transfer in skeletal muscle.lld:pubmed
pubmed-article:16385549pubmed:affiliationDepartment of Molecular Microbiology and Immunology, The University of Missouri, School of Medicine, Columbia, MO 65212, USA.lld:pubmed
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