| pubmed-article:16381046 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16381046 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:16381046 | lifeskim:mentions | umls-concept:C0080188 | lld:lifeskim |
| pubmed-article:16381046 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:16381046 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:16381046 | pubmed:dateCreated | 2006-2-7 | lld:pubmed |
| pubmed-article:16381046 | pubmed:abstractText | Two tetrasaccharides carrying the trisaccharidic Lewis x motif on a GlcNAc or a Gal residue were produced on the gram-scale by high-cell-density cultures of metabolically engineered Escherichia coli strains that overexpressed the Helicobacter pylori futA gene for alpha-3 fucosyltransferase and the Neisseria meningitidis lgtB gene for beta-4 galactosyltransferase. The first compound Galbeta-4(Fucalpha-3)GlcNAcbeta-4GlcNAc was produced by glycosylation of chitinbiose, which was endogenously generated in the bacterial cytoplasm by the successive action of the rhizobial chitin-synthase NodC and the Bacillus circulans chitinase A1, whose genes were additionally expressed in the E. coli strain. The second compound, Galbeta-4(Fucalpha-3)GlcNAcbeta-3Gal, was produced from exogenously added Gal by a strain that was deficient in galactokinase activity and overexpressed the additional N. meningitidis lgtA gene for beta-3 N-acetylglucosaminyltransferase. | lld:pubmed |
| pubmed-article:16381046 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16381046 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16381046 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16381046 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16381046 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16381046 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16381046 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16381046 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:16381046 | pubmed:issn | 1439-4227 | lld:pubmed |
| pubmed-article:16381046 | pubmed:author | pubmed-author:UtilleJean... | lld:pubmed |
| pubmed-article:16381046 | pubmed:author | pubmed-author:HeyraudAlainA | lld:pubmed |
| pubmed-article:16381046 | pubmed:author | pubmed-author:SamainEricE | lld:pubmed |
| pubmed-article:16381046 | pubmed:author | pubmed-author:BossoClaudeC | lld:pubmed |
| pubmed-article:16381046 | pubmed:author | pubmed-author:DumonClaireC | lld:pubmed |
| pubmed-article:16381046 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16381046 | pubmed:volume | 7 | lld:pubmed |
| pubmed-article:16381046 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16381046 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16381046 | pubmed:pagination | 359-65 | lld:pubmed |
| pubmed-article:16381046 | pubmed:meshHeading | pubmed-meshheading:16381046... | lld:pubmed |
| pubmed-article:16381046 | pubmed:meshHeading | pubmed-meshheading:16381046... | lld:pubmed |
| pubmed-article:16381046 | pubmed:meshHeading | pubmed-meshheading:16381046... | lld:pubmed |
| pubmed-article:16381046 | pubmed:meshHeading | pubmed-meshheading:16381046... | lld:pubmed |
| pubmed-article:16381046 | pubmed:meshHeading | pubmed-meshheading:16381046... | lld:pubmed |
| pubmed-article:16381046 | pubmed:meshHeading | pubmed-meshheading:16381046... | lld:pubmed |
| pubmed-article:16381046 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16381046 | pubmed:articleTitle | Production of Lewis x tetrasaccharides by metabolically engineered Escherichia coli. | lld:pubmed |
| pubmed-article:16381046 | pubmed:affiliation | Centre de Recherches sur les Macromolécules Végétales, ICMG, FR CNRS-UJF 2607, B P 53, 38041 Grenoble Cedex 9, France. | lld:pubmed |
| pubmed-article:16381046 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16381046 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16381046 | lld:pubmed |
