| pubmed-article:1637507 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1637507 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:1637507 | lifeskim:mentions | umls-concept:C0004793 | lld:lifeskim |
| pubmed-article:1637507 | lifeskim:mentions | umls-concept:C0542193 | lld:lifeskim |
| pubmed-article:1637507 | lifeskim:mentions | umls-concept:C0243161 | lld:lifeskim |
| pubmed-article:1637507 | lifeskim:mentions | umls-concept:C0205360 | lld:lifeskim |
| pubmed-article:1637507 | lifeskim:mentions | umls-concept:C1707511 | lld:lifeskim |
| pubmed-article:1637507 | lifeskim:mentions | umls-concept:C0205144 | lld:lifeskim |
| pubmed-article:1637507 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:1637507 | pubmed:dateCreated | 1992-9-3 | lld:pubmed |
| pubmed-article:1637507 | pubmed:abstractText | It seems increasingly evident that if the Z form of DNA exists in the genome it must exist as short sections of alternating pyrimidine-purine sequences in the midst of very long sections of B-form DNA. We have determined the minimum length of a string of alternating CG base pairs that can go into the Z form in the middle of a long B form. Self-complimentary oligomers of the form T(M)(CG)(N)A(M) were synthesized. The conformation of the resulting duplex was determined in 6M aqueous NaCl solution by Raman scattering. We have found that 12 alternating CG base pairs is the minimum length required to form a stable Z form of DNA inside of a long B form section. Only the 4 center CG base pairs go into the Z form. These 4 CG base pairs in the Z form are flanked on each side by 4 CG base pairs in a non-Z (probably B) form as well as the ..TT.. ..AA.. sequences in the B form. We propose a model of the B/Z junction in which the double helix flips directly from the B form to the Z form so that there are no base pairs in the junction. In this model the B form is nucleated in the AT base pairs on each end and is propagated into the CG base pairs in the center. This model is supported by isotopic H/D exchange experiments that shows that the H/D exchange of the non-Z form CG base pairs is highly retarded and indicates that they remain in the B form. A Thermodynamic analysis of the concentration dependence of the melting point of the duplexes in both low and high salt, supports our model and rules out the possibility of hairpin formation. The enthalpy for the formation of a B/Z junction is determined to be about +16 kcal/junction. A comparison of these results with recent results on B/Z junctions in super-coiled DNA is given. Molecular modeling calculations permit us to obtain values for the coordinates and torsional angles of the oligomers showing both B/Z and B/Z/B junctions. The Cartesian coordinates for these oligomers as well as stereo figures of these models in color are available from the authors. | lld:pubmed |
| pubmed-article:1637507 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1637507 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1637507 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1637507 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1637507 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1637507 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1637507 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1637507 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:1637507 | pubmed:issn | 0739-1102 | lld:pubmed |
| pubmed-article:1637507 | pubmed:author | pubmed-author:ThomasGG | lld:pubmed |
| pubmed-article:1637507 | pubmed:author | pubmed-author:PeticolasW... | lld:pubmed |
| pubmed-article:1637507 | pubmed:author | pubmed-author:DayJJ | lld:pubmed |
| pubmed-article:1637507 | pubmed:author | pubmed-author:DauchezMM | lld:pubmed |
| pubmed-article:1637507 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1637507 | pubmed:volume | 9 | lld:pubmed |
| pubmed-article:1637507 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1637507 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1637507 | pubmed:pagination | 1155-83 | lld:pubmed |
| pubmed-article:1637507 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:1637507 | pubmed:meshHeading | pubmed-meshheading:1637507-... | lld:pubmed |
| pubmed-article:1637507 | pubmed:meshHeading | pubmed-meshheading:1637507-... | lld:pubmed |
| pubmed-article:1637507 | pubmed:meshHeading | pubmed-meshheading:1637507-... | lld:pubmed |
| pubmed-article:1637507 | pubmed:meshHeading | pubmed-meshheading:1637507-... | lld:pubmed |
| pubmed-article:1637507 | pubmed:meshHeading | pubmed-meshheading:1637507-... | lld:pubmed |
| pubmed-article:1637507 | pubmed:meshHeading | pubmed-meshheading:1637507-... | lld:pubmed |
| pubmed-article:1637507 | pubmed:meshHeading | pubmed-meshheading:1637507-... | lld:pubmed |
| pubmed-article:1637507 | pubmed:meshHeading | pubmed-meshheading:1637507-... | lld:pubmed |
| pubmed-article:1637507 | pubmed:meshHeading | pubmed-meshheading:1637507-... | lld:pubmed |
| pubmed-article:1637507 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1637507 | pubmed:articleTitle | Base sequence criteria and Cartesian coordinates for stable B/Z and B/Z/B junctions in relaxed DNA. | lld:pubmed |
| pubmed-article:1637507 | pubmed:affiliation | Department of Chemistry, University of Oregon, Eugene 97403. | lld:pubmed |
| pubmed-article:1637507 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1637507 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1637507 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1637507 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1637507 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1637507 | lld:pubmed |
