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pubmed-article:16346345pubmed:abstractTextA preparation of two commercial enzymes was used to liberate protoplasts from 16-h-old mycelium of Phanerochaete chrysosporium. Regeneration frequencies of up to 5% were attained when the protoplasts were plated in a medium containing 10% sorbose and 3% agar. Fusion of protoplasts from different auxotrophic strains in polyethylene glycol-Ca produced heterokaryons. Separation of the heterokaryons into their constituent homokaryotic strains could be effected through protoplast release and formation of colonies on regeneration agar.lld:pubmed
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pubmed-article:16346345pubmed:authorpubmed-author:GoldM HMHlld:pubmed
pubmed-article:16346345pubmed:authorpubmed-author:ChengT MTMlld:pubmed
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pubmed-article:16346345pubmed:pagination260-3lld:pubmed
pubmed-article:16346345pubmed:dateRevised2010-9-20lld:pubmed
pubmed-article:16346345pubmed:year1983lld:pubmed
pubmed-article:16346345pubmed:articleTitleFormation, Fusion, and Regeneration of Protoplasts from Wild-Type and Auxotrophic Strains of the White Rot Basidiomycete Phanerochaete chrysosporium.lld:pubmed
pubmed-article:16346345pubmed:affiliationDepartment of Chemistry and Biochemical Sciences, Oregon Graduate Center, Beaverton, Oregon 97006.lld:pubmed
pubmed-article:16346345pubmed:publicationTypeJournal Articlelld:pubmed
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