| pubmed-article:16345400 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16345400 | lifeskim:mentions | umls-concept:C0319600 | lld:lifeskim |
| pubmed-article:16345400 | lifeskim:mentions | umls-concept:C0083183 | lld:lifeskim |
| pubmed-article:16345400 | lifeskim:mentions | umls-concept:C0452720 | lld:lifeskim |
| pubmed-article:16345400 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:16345400 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:16345400 | pubmed:dateCreated | 2010-6-25 | lld:pubmed |
| pubmed-article:16345400 | pubmed:abstractText | Lactase (beta-d-galactosidase) was produced by Candida pseudotropicalis grown in deproteinized whey. Maximum enzyme production in 2% whey was obtained by supplementation with 0.15% yeast extract, 0.1% (NH(4))(2)SO(4), and 0.05% KH(2)PO(4) (wt/vol). Highest enzyme values (4.35 U/mg of cells and 68 U/ml) were obtained with 10 to 12% whey, while enzyme yield was maximal in 2% whey (0.87 U/mg of whey). Optimal initial pH for cultivation was 3.5. The best conditions for extraction included 2% (wt/vol) chloroform, 10 h of treatment, pH 6.6 and higher, and 30 to 37 degrees C. Optimum pH and temperature for enzyme activity were 6.2 and 47 degrees C. The enzyme had a K(m) for O-nitrophenyl-beta-d-galactopyranoside of 3.06 x 10 M and the initial V(max) was estimated as 6.63 x 10 M per min. It hydrolized 50 and 100% of the lactose in whey and milk within 4 and 5 h, respectively, at 37 degrees C. The lyophilized enzyme retained 95% of activity for 3 months when stored at -20 degrees C. | lld:pubmed |
| pubmed-article:16345400 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16345400 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16345400 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16345400 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16345400 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16345400 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16345400 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16345400 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16345400 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16345400 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16345400 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16345400 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16345400 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16345400 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16345400 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16345400 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16345400 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16345400 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16345400 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16345400 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16345400 | pubmed:status | PubMed-not-MEDLINE | lld:pubmed |
| pubmed-article:16345400 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:16345400 | pubmed:issn | 0099-2240 | lld:pubmed |
| pubmed-article:16345400 | pubmed:author | pubmed-author:CastilloF JFJ | lld:pubmed |
| pubmed-article:16345400 | pubmed:author | pubmed-author:de BalesS ASA | lld:pubmed |
| pubmed-article:16345400 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16345400 | pubmed:volume | 37 | lld:pubmed |
| pubmed-article:16345400 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16345400 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16345400 | pubmed:pagination | 1201-5 | lld:pubmed |
| pubmed-article:16345400 | pubmed:dateRevised | 2010-9-20 | lld:pubmed |
| pubmed-article:16345400 | pubmed:year | 1979 | lld:pubmed |
| pubmed-article:16345400 | pubmed:articleTitle | Production of Lactase by Candida pseudotropicalis Grown in Whey. | lld:pubmed |
| pubmed-article:16345400 | pubmed:affiliation | Fermentation Laboratory, Centro de Microbiología y Biología Celular, I.V.I.C., Apartado 1827, Caracas 101, Venezuela. | lld:pubmed |
| pubmed-article:16345400 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16345400 | lld:pubmed |
