| pubmed-article:16338406 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16338406 | lifeskim:mentions | umls-concept:C0872079 | lld:lifeskim |
| pubmed-article:16338406 | lifeskim:mentions | umls-concept:C1254042 | lld:lifeskim |
| pubmed-article:16338406 | lifeskim:mentions | umls-concept:C1510827 | lld:lifeskim |
| pubmed-article:16338406 | lifeskim:mentions | umls-concept:C0678594 | lld:lifeskim |
| pubmed-article:16338406 | lifeskim:mentions | umls-concept:C1527178 | lld:lifeskim |
| pubmed-article:16338406 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:16338406 | pubmed:dateCreated | 2005-12-12 | lld:pubmed |
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| pubmed-article:16338406 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16338406 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16338406 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:16338406 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:16338406 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16338406 | pubmed:abstractText | Although protein-protein interactions are involved in nearly all cellular processes, general rules for describing affinity and selectivity in protein-protein complexes are lacking, primarily because correlations between changes in protein structure and binding energetics have not been well determined. Here, we establish the structural basis of affinity maturation for a protein-protein interaction system that we had previously characterized energetically. This model system exhibits a 1500-fold affinity increase. Also, its affinity maturation is restricted by negative intramolecular cooperativity. With three complex and six unliganded variant X-ray crystal structures, we provide molecular snapshots of protein interface remodeling events that span the breadth of the affinity maturation process and present a comprehensive structural view of affinity maturation. Correlating crystallographically observed structural changes with measured energetic changes reveals molecular bases for affinity maturation, intramolecular cooperativity, and context-dependent binding. | lld:pubmed |
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| pubmed-article:16338406 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16338406 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16338406 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16338406 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16338406 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:16338406 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16338406 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:16338406 | pubmed:issn | 0969-2126 | lld:pubmed |
| pubmed-article:16338406 | pubmed:author | pubmed-author:MariuzzaRoy... | lld:pubmed |
| pubmed-article:16338406 | pubmed:author | pubmed-author:SwaminathanCh... | lld:pubmed |
| pubmed-article:16338406 | pubmed:author | pubmed-author:SundbergEric... | lld:pubmed |
| pubmed-article:16338406 | pubmed:author | pubmed-author:KranzDavid... | lld:pubmed |
| pubmed-article:16338406 | pubmed:author | pubmed-author:GuanRongjinR | lld:pubmed |
| pubmed-article:16338406 | pubmed:author | pubmed-author:YangJianyingJ | lld:pubmed |
| pubmed-article:16338406 | pubmed:author | pubmed-author:ChoSangwooS | lld:pubmed |
| pubmed-article:16338406 | pubmed:author | pubmed-author:KiekeMichele... | lld:pubmed |
| pubmed-article:16338406 | pubmed:author | pubmed-author:KerzicMelissa... | lld:pubmed |
| pubmed-article:16338406 | pubmed:issnType | Print | lld:pubmed |
