pubmed-article:1629340 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:1629340 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
pubmed-article:1629340 | lifeskim:mentions | umls-concept:C0580797 | lld:lifeskim |
pubmed-article:1629340 | lifeskim:mentions | umls-concept:C0020202 | lld:lifeskim |
pubmed-article:1629340 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
pubmed-article:1629340 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
pubmed-article:1629340 | lifeskim:mentions | umls-concept:C0003339 | lld:lifeskim |
pubmed-article:1629340 | lifeskim:mentions | umls-concept:C1521761 | lld:lifeskim |
pubmed-article:1629340 | lifeskim:mentions | umls-concept:C1524063 | lld:lifeskim |
pubmed-article:1629340 | lifeskim:mentions | umls-concept:C0056135 | lld:lifeskim |
pubmed-article:1629340 | pubmed:issue | 7 | lld:pubmed |
pubmed-article:1629340 | pubmed:dateCreated | 1992-8-20 | lld:pubmed |
pubmed-article:1629340 | pubmed:abstractText | We developed an accurate nonradioactive colony hybridization assay (NCHA) using a digoxigenin-labeled polynucleotide probe and an antidigoxigenin alkaline phosphatase conjugate for the identification of enterotoxigenic Escherichia coli (ETEC) harboring genes for colonization factor antigen I (CFA/I), coli surface antigen 4 (CS4), or putative colonization factor O166 (PCFO166). In this 2-day assay, visual registration of color intensity could be used to distinguish between CFA/I-positive strains and strains with the genetic potential to express CS4 or PCFO166. A rapid NCHA was developed by which the results could be read visually 7 h and 45 min after inoculation of the bacteria. In the rapid NCHA, densitometry verified the visual discrimination between four groups of E. coli; ETEC with the CFA/I gene, ETEC with the CS4 gene, ETEC with the PCFO166 gene, and E. coli strains that lack such genes. As a confirmatory test, plasmids from ETEC with the CFA/I, CS4, or PCFO166 gene were differentiated by their characteristic restriction fragment patterns in nonradioactive Southern blot hybridization. | lld:pubmed |
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pubmed-article:1629340 | pubmed:language | eng | lld:pubmed |
pubmed-article:1629340 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1629340 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:1629340 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:1629340 | pubmed:month | Jul | lld:pubmed |
pubmed-article:1629340 | pubmed:issn | 0095-1137 | lld:pubmed |
pubmed-article:1629340 | pubmed:author | pubmed-author:GaastraWW | lld:pubmed |
pubmed-article:1629340 | pubmed:author | pubmed-author:BoveJ MJM | lld:pubmed |
pubmed-article:1629340 | pubmed:author | pubmed-author:SvennerholmA... | lld:pubmed |
pubmed-article:1629340 | pubmed:author | pubmed-author:SommerfeltHH | lld:pubmed |
pubmed-article:1629340 | pubmed:author | pubmed-author:GrewalH MHM | lld:pubmed |
pubmed-article:1629340 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:1629340 | pubmed:volume | 30 | lld:pubmed |
pubmed-article:1629340 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:1629340 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:1629340 | pubmed:pagination | 1823-8 | lld:pubmed |
pubmed-article:1629340 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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pubmed-article:1629340 | pubmed:meshHeading | pubmed-meshheading:1629340-... | lld:pubmed |
pubmed-article:1629340 | pubmed:year | 1992 | lld:pubmed |
pubmed-article:1629340 | pubmed:articleTitle | Use of nonradioactive DNA hybridization for identification of enterotoxigenic Escherichia coli harboring genes for colonization factor antigen I, coli surface antigen 4, or putative colonization factor O166. | lld:pubmed |
pubmed-article:1629340 | pubmed:affiliation | Centre for International Health, University of Bergen, Haukeland Hospital, Norway. | lld:pubmed |
pubmed-article:1629340 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:1629340 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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