| pubmed-article:16277834 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16277834 | lifeskim:mentions | umls-concept:C0025202 | lld:lifeskim |
| pubmed-article:16277834 | lifeskim:mentions | umls-concept:C0003320 | lld:lifeskim |
| pubmed-article:16277834 | lifeskim:mentions | umls-concept:C0600432 | lld:lifeskim |
| pubmed-article:16277834 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:16277834 | lifeskim:mentions | umls-concept:C2610698 | lld:lifeskim |
| pubmed-article:16277834 | lifeskim:mentions | umls-concept:C0237881 | lld:lifeskim |
| pubmed-article:16277834 | lifeskim:mentions | umls-concept:C0750502 | lld:lifeskim |
| pubmed-article:16277834 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:16277834 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:16277834 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:16277834 | pubmed:dateCreated | 2005-11-9 | lld:pubmed |
| pubmed-article:16277834 | pubmed:abstractText | This study was aimed to explore the expression and significance of melanoma antigen gene-3 (MAGE-3) in endoplasmic reticulum stress-induced apoptosis. After the treatment of leukemia cell line K562 and its multidrug-resistant cell line K562/A02 by thapsigargin, intracellular calcium concentrations ([Ca(2+)]i) in K562 and K562/A02 were measured by fluorescence spectrophotometer with calcium sensitive fluorescence indicator Fura-2/AM; expression changes of glucose-regulated protein 78 (GRP78) were detected by Western blot; morphological change of apoptotic cell was investigated by AO/EB fluorescent staining under fluorescent microscope; apoptosis rate was determined by terminal deoxyribonucleotidyl transferase (TdT)-mediated dUTP nick end labelling (TUNEL) staining; the expression of MAGE-3 gene mRNA was detected by RT-PCR. The results showed that (1) thapsigargin induced the enhancement of [Ca(2+)]i with different extent in K562 and K562/A02 cells, and the enhancement of [Ca(2+)]i was dose-dependent in experiment range. At the same time, thapsigargin induced upregulation of GRP78 protein expression and typical apoptotic changes of K562 and K562/A02 cells, apoptotic rate was also dose-dependent in experiment range. The [Ca(2+)]i in K562/A02 cells were higher than that in K562 cells. (2) in the course of endoplasmic reticulum stress-induced apoptosis by thapsigargin, the expression of MAGE-3 gene mRNA was remarkably downregulated. Moreover, the expression of MAGE-3 gene mRNA in K562/A02 cells was higher than that in K562 cells. It is concluded that (1) thapsigargin induces endoplasmic reticulum stress-induced apoptosis of K562 and K562/A02 cells in experiment range, and this may be associated with downregulation of MAGE-3 mRNA expression or MAGE-3 gene may participates in the regulation of endoplasmic reticulum stress-induced apoptosis. (2) MAGE-3 gene may possess anti-apoptotic activity, multidrug resistance in K562/A02 cells can be associated with [Ca(2+)]i increase and upregulation of MAGE-3 expression. | lld:pubmed |
| pubmed-article:16277834 | pubmed:language | chi | lld:pubmed |
| pubmed-article:16277834 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16277834 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16277834 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16277834 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16277834 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16277834 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16277834 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16277834 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16277834 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16277834 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16277834 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16277834 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16277834 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:16277834 | pubmed:issn | 1009-2137 | lld:pubmed |
| pubmed-article:16277834 | pubmed:author | pubmed-author:ZhaoD HDH | lld:pubmed |
| pubmed-article:16277834 | pubmed:author | pubmed-author:DadoDD | lld:pubmed |
| pubmed-article:16277834 | pubmed:author | pubmed-author:ZouPingP | lld:pubmed |
| pubmed-article:16277834 | pubmed:author | pubmed-author:XiaoJuanJ | lld:pubmed |
| pubmed-article:16277834 | pubmed:author | pubmed-author:FengXian-QiXQ | lld:pubmed |
| pubmed-article:16277834 | pubmed:author | pubmed-author:NieShu-MinSM | lld:pubmed |
| pubmed-article:16277834 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16277834 | pubmed:volume | 13 | lld:pubmed |
| pubmed-article:16277834 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16277834 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16277834 | pubmed:pagination | 741-5 | lld:pubmed |
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| pubmed-article:16277834 | pubmed:meshHeading | pubmed-meshheading:16277834... | lld:pubmed |
| pubmed-article:16277834 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:16277834 | pubmed:articleTitle | [Expression and significance of melanoma antigen gene-3 in endoplasmic reticulum stress-induced apoptosis of K562 cells]. | lld:pubmed |
| pubmed-article:16277834 | pubmed:affiliation | Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. fxqnsm2000@yahoo.com.cn | lld:pubmed |
| pubmed-article:16277834 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16277834 | pubmed:publicationType | English Abstract | lld:pubmed |
| pubmed-article:16277834 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
