| pubmed-article:16272752 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16272752 | lifeskim:mentions | umls-concept:C0009676 | lld:lifeskim |
| pubmed-article:16272752 | lifeskim:mentions | umls-concept:C1332830 | lld:lifeskim |
| pubmed-article:16272752 | lifeskim:mentions | umls-concept:C1707227 | lld:lifeskim |
| pubmed-article:16272752 | lifeskim:mentions | umls-concept:C1285573 | lld:lifeskim |
| pubmed-article:16272752 | lifeskim:mentions | umls-concept:C0678594 | lld:lifeskim |
| pubmed-article:16272752 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:16272752 | lifeskim:mentions | umls-concept:C1547301 | lld:lifeskim |
| pubmed-article:16272752 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:16272752 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:16272752 | pubmed:dateCreated | 2005-11-7 | lld:pubmed |
| pubmed-article:16272752 | pubmed:abstractText | We encountered DNA samples which showed a positive product using a long PCR-based method for the detection of CYP2D6*5, indicating deletion of the entire CYP2D6 gene, but the samples did not show a band related to CYP2D6*5 in either XbaI- or EcoRI-RFLP analysis. To achieve genotyping with accuracy, we performed a further genetic analysis to clarify the discrepancy. An unknown 1.6-kb insert was identified in a region downstream from the CYP2D6 stop codon where a specific primer was designed for long-PCR analysis for CYP2D6*5 genotyping. This finding suggested that the CYP2D6 gene might not be deleted in the samples even if a positive product was detected by the long-PCR method. Furthermore, the allelic frequency of this type was found to be approximately 0.3% (4 heterozygous/771 samples) in a Japanese population. In conclusion, we found a novel structure of the CYP2D6 gene, which might lead to incorrect genotyping for CYP2D6*5. Although the long PCR-based strategy for the detection of CYP2D6*5 has been widely used due to its usefulness and convenience, we recommend caution when adopting this method and propose re-evaluating the method for detecting CYP2D6*5. | lld:pubmed |
| pubmed-article:16272752 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16272752 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16272752 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16272752 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16272752 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16272752 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16272752 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:16272752 | pubmed:issn | 1347-4367 | lld:pubmed |
| pubmed-article:16272752 | pubmed:author | pubmed-author:FukudaKazuhir... | lld:pubmed |
| pubmed-article:16272752 | pubmed:author | pubmed-author:FukudaTsuyosh... | lld:pubmed |
| pubmed-article:16272752 | pubmed:author | pubmed-author:MauneHiromiH | lld:pubmed |
| pubmed-article:16272752 | pubmed:author | pubmed-author:IkenagaYukaY | lld:pubmed |
| pubmed-article:16272752 | pubmed:author | pubmed-author:AzumaJunichiJ | lld:pubmed |
| pubmed-article:16272752 | pubmed:author | pubmed-author:NaoharaMasaka... | lld:pubmed |
| pubmed-article:16272752 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16272752 | pubmed:volume | 20 | lld:pubmed |
| pubmed-article:16272752 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16272752 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16272752 | pubmed:pagination | 345-50 | lld:pubmed |
| pubmed-article:16272752 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:16272752 | pubmed:meshHeading | pubmed-meshheading:16272752... | lld:pubmed |
| pubmed-article:16272752 | pubmed:meshHeading | pubmed-meshheading:16272752... | lld:pubmed |
| pubmed-article:16272752 | pubmed:meshHeading | pubmed-meshheading:16272752... | lld:pubmed |
| pubmed-article:16272752 | pubmed:meshHeading | pubmed-meshheading:16272752... | lld:pubmed |
| pubmed-article:16272752 | pubmed:meshHeading | pubmed-meshheading:16272752... | lld:pubmed |
| pubmed-article:16272752 | pubmed:meshHeading | pubmed-meshheading:16272752... | lld:pubmed |
| pubmed-article:16272752 | pubmed:meshHeading | pubmed-meshheading:16272752... | lld:pubmed |
| pubmed-article:16272752 | pubmed:meshHeading | pubmed-meshheading:16272752... | lld:pubmed |
| pubmed-article:16272752 | pubmed:meshHeading | pubmed-meshheading:16272752... | lld:pubmed |
| pubmed-article:16272752 | pubmed:meshHeading | pubmed-meshheading:16272752... | lld:pubmed |
| pubmed-article:16272752 | pubmed:meshHeading | pubmed-meshheading:16272752... | lld:pubmed |
| pubmed-article:16272752 | pubmed:meshHeading | pubmed-meshheading:16272752... | lld:pubmed |
| pubmed-article:16272752 | pubmed:meshHeading | pubmed-meshheading:16272752... | lld:pubmed |
| pubmed-article:16272752 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:16272752 | pubmed:articleTitle | Novel structure of the CYP2D6 gene that confuses genotyping for the CYP2D6*5 allele. | lld:pubmed |
| pubmed-article:16272752 | pubmed:affiliation | Clinical Evaluation of Medicines and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Yamada-oka, Osaka, Japan. | lld:pubmed |
| pubmed-article:16272752 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16272752 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:16272752 | pubmed:publicationType | Evaluation Studies | lld:pubmed |
| entrez-gene:1565 | entrezgene:pubmed | pubmed-article:16272752 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:16272752 | lld:entrezgene |
