| pubmed-article:16249066 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16249066 | lifeskim:mentions | umls-concept:C0441635 | lld:lifeskim |
| pubmed-article:16249066 | lifeskim:mentions | umls-concept:C0017429 | lld:lifeskim |
| pubmed-article:16249066 | lifeskim:mentions | umls-concept:C0599894 | lld:lifeskim |
| pubmed-article:16249066 | lifeskim:mentions | umls-concept:C1511693 | lld:lifeskim |
| pubmed-article:16249066 | lifeskim:mentions | umls-concept:C1521840 | lld:lifeskim |
| pubmed-article:16249066 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:16249066 | pubmed:dateCreated | 2005-12-12 | lld:pubmed |
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| pubmed-article:16249066 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16249066 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16249066 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16249066 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16249066 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16249066 | pubmed:abstractText | Currently, challenges exist to acquire long-range (hundreds of kilobase pairs) phase-discriminated sequence across substantial numbers of individuals. We have developed a straightforward method for isolating and characterizing specific genomic regions in a haplospecific manner. Real-time PCR is carried out to STS content map and genotype pools of fosmid clones arrayed in 384-well microtiter plates. Single-nucleotide polymorphisms, microsatellite markers, and insertion-deletion polymorphisms are used to differentiate the target region into haplotype-specific tiling paths. DNA of clones from these tiling paths is retrieved from the library and either sequenced by standard shotgun methods or amplified in vitro and sequenced by a primer-based, directed method. This approach provides convenient access to complete, haplotype-resolved resequencing data from multiple individuals across tens to hundreds of thousands of basepairs. We illustrate its implementation with a detailed example of more than 400 kbp from the human CFTR region, across 15 individuals, and summarize our experience applying it to many other human loci. | lld:pubmed |
| pubmed-article:16249066 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16249066 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16249066 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16249066 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16249066 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16249066 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:16249066 | pubmed:issn | 0888-7543 | lld:pubmed |
| pubmed-article:16249066 | pubmed:author | pubmed-author:RaymondChrist... | lld:pubmed |
| pubmed-article:16249066 | pubmed:author | pubmed-author:OlsonMaynard... | lld:pubmed |
| pubmed-article:16249066 | pubmed:author | pubmed-author:KaulRajinderR | lld:pubmed |
| pubmed-article:16249066 | pubmed:author | pubmed-author:ChangJeanJ | lld:pubmed |
| pubmed-article:16249066 | pubmed:author | pubmed-author:PaddockMarcia... | lld:pubmed |
| pubmed-article:16249066 | pubmed:author | pubmed-author:WaringDavidD | lld:pubmed |
| pubmed-article:16249066 | pubmed:author | pubmed-author:SubramanianSa... | lld:pubmed |
| pubmed-article:16249066 | pubmed:author | pubmed-author:HaugenEricE | lld:pubmed |
| pubmed-article:16249066 | pubmed:author | pubmed-author:BoveeDonaldD | lld:pubmed |
| pubmed-article:16249066 | pubmed:author | pubmed-author:QiuRuolanR | lld:pubmed |
| pubmed-article:16249066 | pubmed:author | pubmed-author:PalmieriAntho... | lld:pubmed |
| pubmed-article:16249066 | pubmed:author | pubmed-author:DeodatoChloeC | lld:pubmed |
| pubmed-article:16249066 | pubmed:author | pubmed-author:RadkeTanaT | lld:pubmed |
| pubmed-article:16249066 | pubmed:author | pubmed-author:KasArnieA | lld:pubmed |
| pubmed-article:16249066 | pubmed:author | pubmed-author:StacyRobinR | lld:pubmed |
| pubmed-article:16249066 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16249066 | pubmed:volume | 86 | lld:pubmed |
| pubmed-article:16249066 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16249066 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16249066 | pubmed:pagination | 759-66 | lld:pubmed |
| pubmed-article:16249066 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:16249066 | pubmed:meshHeading | pubmed-meshheading:16249066... | lld:pubmed |
| pubmed-article:16249066 | pubmed:meshHeading | pubmed-meshheading:16249066... | lld:pubmed |
| pubmed-article:16249066 | pubmed:meshHeading | pubmed-meshheading:16249066... | lld:pubmed |
| pubmed-article:16249066 | pubmed:meshHeading | pubmed-meshheading:16249066... | lld:pubmed |
| pubmed-article:16249066 | pubmed:meshHeading | pubmed-meshheading:16249066... | lld:pubmed |
| pubmed-article:16249066 | pubmed:meshHeading | pubmed-meshheading:16249066... | lld:pubmed |
| pubmed-article:16249066 | pubmed:meshHeading | pubmed-meshheading:16249066... | lld:pubmed |
| pubmed-article:16249066 | pubmed:meshHeading | pubmed-meshheading:16249066... | lld:pubmed |
| pubmed-article:16249066 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:16249066 | pubmed:articleTitle | Targeted, haplotype-resolved resequencing of long segments of the human genome. | lld:pubmed |
| pubmed-article:16249066 | pubmed:affiliation | University of Washington Genome Center, Department of Medicine, University of Washington, Seattle, WA 98195, USA. | lld:pubmed |
| pubmed-article:16249066 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16249066 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:16249066 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:16249066 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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