| pubmed-article:1624793 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1624793 | lifeskim:mentions | umls-concept:C0004037 | lld:lifeskim |
| pubmed-article:1624793 | lifeskim:mentions | umls-concept:C0002092 | lld:lifeskim |
| pubmed-article:1624793 | lifeskim:mentions | umls-concept:C0332307 | lld:lifeskim |
| pubmed-article:1624793 | lifeskim:mentions | umls-concept:C0009015 | lld:lifeskim |
| pubmed-article:1624793 | lifeskim:mentions | umls-concept:C0037296 | lld:lifeskim |
| pubmed-article:1624793 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:1624793 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:1624793 | lifeskim:mentions | umls-concept:C1149309 | lld:lifeskim |
| pubmed-article:1624793 | lifeskim:mentions | umls-concept:C0995147 | lld:lifeskim |
| pubmed-article:1624793 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:1624793 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:1624793 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:1624793 | pubmed:dateCreated | 1992-8-11 | lld:pubmed |
| pubmed-article:1624793 | pubmed:abstractText | Aspergillus fumigatus secretes an 18-kDa nonglycosylated IgE-binding protein. This protein was previously shown to be a ribotoxin, like alpha-sarcin and mitogillin. A 686-bp long A. fumigatus cDNA encoding an 18-kDa ribotoxin was cloned and expressed in Escherichia coli as a fusion protein with six adjacent histidines (rAsp f I/a). rAsp f I/a was purified to homogeneity by Ni(2+)-chelate affinity chromatography and refolded. The recombinant protein was enzymatically active resulting in the cleavage of 28S rRNA within a universally conserved region. rAsp f I/a was cytotoxic for EBV immortalized or PHA stimulated human PBMC. Furthermore, rAsp f I/a was recognized by murine mAb made against an 18-kDa ribotoxin. IgE of individuals allergic to A. fumigatus bound to rAsp f I/a as shown by ELISA, dot blots, and Western blots. rAsp f I/a elicited positive immediate type I skin reactions in individuals allergic to A. fumigatus but not in healthy control individuals. The results show that rAsp f I/a has similar functional characteristics when compared to the native 18-kDa ribotoxin. rAsp f I/a expressed in E. coli can therefore be used as a standardized Ag/allergen for serologic and clinical diagnosis of A. fumigatus-associated diseases. | lld:pubmed |
| pubmed-article:1624793 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1624793 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1624793 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:1624793 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:1624793 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1624793 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:1624793 | pubmed:issn | 0022-1767 | lld:pubmed |
| pubmed-article:1624793 | pubmed:author | pubmed-author:BlaserKK | lld:pubmed |
| pubmed-article:1624793 | pubmed:author | pubmed-author:VirchowCC | lld:pubmed |
| pubmed-article:1624793 | pubmed:author | pubmed-author:MosesLL | lld:pubmed |
| pubmed-article:1624793 | pubmed:author | pubmed-author:SchneiderTT | lld:pubmed |
| pubmed-article:1624793 | pubmed:author | pubmed-author:SuterMM | lld:pubmed |
| pubmed-article:1624793 | pubmed:author | pubmed-author:MenzGG | lld:pubmed |
| pubmed-article:1624793 | pubmed:author | pubmed-author:CrameriRR | lld:pubmed |
| pubmed-article:1624793 | pubmed:author | pubmed-author:GmachlMM | lld:pubmed |
| pubmed-article:1624793 | pubmed:author | pubmed-author:DudlerTT | lld:pubmed |
| pubmed-article:1624793 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1624793 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:1624793 | pubmed:volume | 149 | lld:pubmed |
| pubmed-article:1624793 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1624793 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1624793 | pubmed:pagination | 454-60 | lld:pubmed |
| pubmed-article:1624793 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:1624793 | pubmed:meshHeading | pubmed-meshheading:1624793-... | lld:pubmed |
| pubmed-article:1624793 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1624793 | pubmed:articleTitle | Cloning and expression of recombinant Aspergillus fumigatus allergen I/a (rAsp f I/a) with IgE binding and type I skin test activity. | lld:pubmed |
| pubmed-article:1624793 | pubmed:affiliation | Swiss Institute of Allergy and Asthma Research (SIAF), Davos. | lld:pubmed |
| pubmed-article:1624793 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1624793 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:1624793 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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