| pubmed-article:16233814 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16233814 | lifeskim:mentions | umls-concept:C0995754 | lld:lifeskim |
| pubmed-article:16233814 | lifeskim:mentions | umls-concept:C1295886 | lld:lifeskim |
| pubmed-article:16233814 | lifeskim:mentions | umls-concept:C0009015 | lld:lifeskim |
| pubmed-article:16233814 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:16233814 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:16233814 | lifeskim:mentions | umls-concept:C1561491 | lld:lifeskim |
| pubmed-article:16233814 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:16233814 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:16233814 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:16233814 | pubmed:dateCreated | 2005-10-19 | lld:pubmed |
| pubmed-article:16233814 | pubmed:abstractText | A gene encoding exoglucanase (CBHII) of Streptomyces sp. M 23 was cloned and sequenced. The cbhII gene consisted of 1359 bp capable of encoding a polypeptide of 453 amino acids with a calculated molecular mass of 45,175 Da. The deduced amino acid sequence showed homology with those of cellulases belonging to family 6 of the glycosyl hydrolases. The cbhII gene was subcloned into the plasmid pSEV1 and expressed in Streptomyces lividans TK-24. The transformed cells were able to secrete the enzyme efficiently in an active form. The CBHII expressed by S. lividans TK-24 was purified to homogeneity by SDS-polyacrylamide gel and characterized. The recombinant CBHII was stable up to 50 degrees C and more than 30% of the original activity remained after heating at 100 degrees C for 10 min. The amino-terminal amino acid sequence of the recombinant CBHII was identified as GPAAPTARVD. These results agreed well with the properties of the authentic CBHII. | lld:pubmed |
| pubmed-article:16233814 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16233814 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16233814 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16233814 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16233814 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16233814 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16233814 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:16233814 | pubmed:issn | 1389-1723 | lld:pubmed |
| pubmed-article:16233814 | pubmed:author | pubmed-author:TakadaGoroG | lld:pubmed |
| pubmed-article:16233814 | pubmed:author | pubmed-author:IzumoriKenK | lld:pubmed |
| pubmed-article:16233814 | pubmed:author | pubmed-author:KawaguchiTaka... | lld:pubmed |
| pubmed-article:16233814 | pubmed:author | pubmed-author:AraiMotooM | lld:pubmed |
| pubmed-article:16233814 | pubmed:author | pubmed-author:SumitaniJun-I... | lld:pubmed |
| pubmed-article:16233814 | pubmed:author | pubmed-author:ParkChang-SuC... | lld:pubmed |
| pubmed-article:16233814 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16233814 | pubmed:volume | 99 | lld:pubmed |
| pubmed-article:16233814 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16233814 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16233814 | pubmed:pagination | 434-6 | lld:pubmed |
| pubmed-article:16233814 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:16233814 | pubmed:meshHeading | pubmed-meshheading:16233814... | lld:pubmed |
| pubmed-article:16233814 | pubmed:meshHeading | pubmed-meshheading:16233814... | lld:pubmed |
| pubmed-article:16233814 | pubmed:meshHeading | pubmed-meshheading:16233814... | lld:pubmed |
| pubmed-article:16233814 | pubmed:meshHeading | pubmed-meshheading:16233814... | lld:pubmed |
| pubmed-article:16233814 | pubmed:meshHeading | pubmed-meshheading:16233814... | lld:pubmed |
| pubmed-article:16233814 | pubmed:meshHeading | pubmed-meshheading:16233814... | lld:pubmed |
| pubmed-article:16233814 | pubmed:meshHeading | pubmed-meshheading:16233814... | lld:pubmed |
| pubmed-article:16233814 | pubmed:meshHeading | pubmed-meshheading:16233814... | lld:pubmed |
| pubmed-article:16233814 | pubmed:meshHeading | pubmed-meshheading:16233814... | lld:pubmed |
| pubmed-article:16233814 | pubmed:meshHeading | pubmed-meshheading:16233814... | lld:pubmed |
| pubmed-article:16233814 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:16233814 | pubmed:articleTitle | Cloning and sequencing of an exoglucanase gene from Streptomyces sp. M 23, and its expression in Streptomyces lividans TK-24. | lld:pubmed |
| pubmed-article:16233814 | pubmed:affiliation | Division of Applied Biological Chemistry, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan. | lld:pubmed |
| pubmed-article:16233814 | pubmed:publicationType | Journal Article | lld:pubmed |
