| pubmed-article:16226432 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16226432 | lifeskim:mentions | umls-concept:C0004611 | lld:lifeskim |
| pubmed-article:16226432 | lifeskim:mentions | umls-concept:C0018150 | lld:lifeskim |
| pubmed-article:16226432 | lifeskim:mentions | umls-concept:C0279516 | lld:lifeskim |
| pubmed-article:16226432 | lifeskim:mentions | umls-concept:C1554184 | lld:lifeskim |
| pubmed-article:16226432 | lifeskim:mentions | umls-concept:C0441712 | lld:lifeskim |
| pubmed-article:16226432 | lifeskim:mentions | umls-concept:C0132537 | lld:lifeskim |
| pubmed-article:16226432 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:16226432 | pubmed:dateCreated | 2005-10-24 | lld:pubmed |
| pubmed-article:16226432 | pubmed:abstractText | Nisin, an amphipathic antibiotic peptide, is produced by a number of strains of Lactococcus lactis subsp. lactis. It has been employed as a food preservative as it has a high antibacterial activity with a relatively low toxicity for humans. Nisin is known to exert a high antibacterial activity against Gram-positive but not Gram-negative bacteria. However, purified nisin Z was found to show an antibacterial activity both against Gram-positive and Gram-negative bacteria. To clarify the mechanisms of activity, nisin Z and purified nisin Z were tested for their antibacterial activities in a high-salt environment. The activity of nisin Z against Staphylococcus aureus was stable even in the presence of NaCl at 100 mM, showing ca. 2log colony-forming unit (CFU) reduction. In contrast, the activity of nisin Z against Escherichia coli was highly sensitive to the same concentration of NaCl, and CFU reduction was not observed. Furthermore, purified nisin Z caused the permeabilisation both of S. aureus and E. coli cytoplasmic membranes. The permeabilisation of E. coli but not S. aureus cytoplasmic membranes was remarkably reduced in a high-salt environment. Moreover, vancomycin inhibited the nisin Z-induced permeabilisation of the S. aureus cytoplasmic membrane. These results suggest that nisin Z utilises two distinct mechanisms of antibacterial activity: a high-salt-sensitive mechanism for E. coli and a high-salt-insensitive mechanism for S. aureus. | lld:pubmed |
| pubmed-article:16226432 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16226432 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16226432 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16226432 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16226432 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16226432 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16226432 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16226432 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16226432 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16226432 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:16226432 | pubmed:issn | 0924-8579 | lld:pubmed |
| pubmed-article:16226432 | pubmed:author | pubmed-author:ShimizuTakash... | lld:pubmed |
| pubmed-article:16226432 | pubmed:author | pubmed-author:TanakaNorikoN | lld:pubmed |
| pubmed-article:16226432 | pubmed:author | pubmed-author:SonomotoKenji... | lld:pubmed |
| pubmed-article:16226432 | pubmed:author | pubmed-author:KuwanoKoichiK | lld:pubmed |
| pubmed-article:16226432 | pubmed:author | pubmed-author:NouShinsukeS | lld:pubmed |
| pubmed-article:16226432 | pubmed:author | pubmed-author:NagatoshiKohe... | lld:pubmed |
| pubmed-article:16226432 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16226432 | pubmed:volume | 26 | lld:pubmed |
| pubmed-article:16226432 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16226432 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16226432 | pubmed:pagination | 396-402 | lld:pubmed |
| pubmed-article:16226432 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:16226432 | pubmed:meshHeading | pubmed-meshheading:16226432... | lld:pubmed |
| pubmed-article:16226432 | pubmed:meshHeading | pubmed-meshheading:16226432... | lld:pubmed |
| pubmed-article:16226432 | pubmed:meshHeading | pubmed-meshheading:16226432... | lld:pubmed |
| pubmed-article:16226432 | pubmed:meshHeading | pubmed-meshheading:16226432... | lld:pubmed |
| pubmed-article:16226432 | pubmed:meshHeading | pubmed-meshheading:16226432... | lld:pubmed |
| pubmed-article:16226432 | pubmed:meshHeading | pubmed-meshheading:16226432... | lld:pubmed |
| pubmed-article:16226432 | pubmed:meshHeading | pubmed-meshheading:16226432... | lld:pubmed |
| pubmed-article:16226432 | pubmed:meshHeading | pubmed-meshheading:16226432... | lld:pubmed |
| pubmed-article:16226432 | pubmed:meshHeading | pubmed-meshheading:16226432... | lld:pubmed |
| pubmed-article:16226432 | pubmed:meshHeading | pubmed-meshheading:16226432... | lld:pubmed |
| pubmed-article:16226432 | pubmed:meshHeading | pubmed-meshheading:16226432... | lld:pubmed |
| pubmed-article:16226432 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:16226432 | pubmed:articleTitle | Dual antibacterial mechanisms of nisin Z against Gram-positive and Gram-negative bacteria. | lld:pubmed |
| pubmed-article:16226432 | pubmed:affiliation | Department of Bacteriology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka 830-0011, Japan. kuwano@med.kurume-u.ac.jp | lld:pubmed |
| pubmed-article:16226432 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16226432 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:16226432 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16226432 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16226432 | lld:pubmed |
