| pubmed-article:1618983 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1618983 | lifeskim:mentions | umls-concept:C0002092 | lld:lifeskim |
| pubmed-article:1618983 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:1618983 | lifeskim:mentions | umls-concept:C0026231 | lld:lifeskim |
| pubmed-article:1618983 | lifeskim:mentions | umls-concept:C0204727 | lld:lifeskim |
| pubmed-article:1618983 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:1618983 | lifeskim:mentions | umls-concept:C1511780 | lld:lifeskim |
| pubmed-article:1618983 | lifeskim:mentions | umls-concept:C1707455 | lld:lifeskim |
| pubmed-article:1618983 | lifeskim:mentions | umls-concept:C0520510 | lld:lifeskim |
| pubmed-article:1618983 | lifeskim:mentions | umls-concept:C1122992 | lld:lifeskim |
| pubmed-article:1618983 | lifeskim:mentions | umls-concept:C1301820 | lld:lifeskim |
| pubmed-article:1618983 | lifeskim:mentions | umls-concept:C2828366 | lld:lifeskim |
| pubmed-article:1618983 | lifeskim:mentions | umls-concept:C0205164 | lld:lifeskim |
| pubmed-article:1618983 | lifeskim:mentions | umls-concept:C1705851 | lld:lifeskim |
| pubmed-article:1618983 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:1618983 | lifeskim:mentions | umls-concept:C1555707 | lld:lifeskim |
| pubmed-article:1618983 | lifeskim:mentions | umls-concept:C2752151 | lld:lifeskim |
| pubmed-article:1618983 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:1618983 | pubmed:dateCreated | 1992-8-5 | lld:pubmed |
| pubmed-article:1618983 | pubmed:abstractText | A high degree of purity is a prerequisite for an allergen preparation to be suitable for clinical diagnosis and therapy. A pure allergen can easily be obtained from a crude mite culture extract by using an immunosorbent prepared with highly specific monoclonal antibodies or from a cDNA-coded material. However, up to now none of these methods has been performed on a process scale. Here large-scale purification is defined as a process in which a crude Dermatophagoides pteronyssinus mite culture extract is essentially fractionated by acetone and ammonium sulphate precipitations followed by anion-exchange high-performance liquid chromatography. A high yield of a very pure Der pI allergen is obtained during the first isocratic run, as shown by sodium dodecylsulphate-polyacrylamide gel electrophoresis, capillary electrophoresis, chromatofocusing and a two site monoclonal antibody enzyme-linked immunosorbent assay. Microsequencing revealed that the 25-residue sequence obtained is entirely in agreement with the sequence derived from the cDNA of Der pI. | lld:pubmed |
| pubmed-article:1618983 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1618983 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1618983 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1618983 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1618983 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1618983 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1618983 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1618983 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1618983 | pubmed:month | May | lld:pubmed |
| pubmed-article:1618983 | pubmed:issn | 0021-9673 | lld:pubmed |
| pubmed-article:1618983 | pubmed:author | pubmed-author:DavidBB | lld:pubmed |
| pubmed-article:1618983 | pubmed:author | pubmed-author:DandeuJ PJP | lld:pubmed |
| pubmed-article:1618983 | pubmed:author | pubmed-author:LuxMM | lld:pubmed |
| pubmed-article:1618983 | pubmed:author | pubmed-author:RabillonJJ | lld:pubmed |
| pubmed-article:1618983 | pubmed:author | pubmed-author:CamoinLL | lld:pubmed |
| pubmed-article:1618983 | pubmed:author | pubmed-author:GuillaumeJ... | lld:pubmed |
| pubmed-article:1618983 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1618983 | pubmed:day | 22 | lld:pubmed |
| pubmed-article:1618983 | pubmed:volume | 599 | lld:pubmed |
| pubmed-article:1618983 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1618983 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1618983 | pubmed:pagination | 105-11 | lld:pubmed |
| pubmed-article:1618983 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:1618983 | pubmed:meshHeading | pubmed-meshheading:1618983-... | lld:pubmed |
| pubmed-article:1618983 | pubmed:meshHeading | pubmed-meshheading:1618983-... | lld:pubmed |
| pubmed-article:1618983 | pubmed:meshHeading | pubmed-meshheading:1618983-... | lld:pubmed |
| pubmed-article:1618983 | pubmed:meshHeading | pubmed-meshheading:1618983-... | lld:pubmed |
| pubmed-article:1618983 | pubmed:meshHeading | pubmed-meshheading:1618983-... | lld:pubmed |
| pubmed-article:1618983 | pubmed:meshHeading | pubmed-meshheading:1618983-... | lld:pubmed |
| pubmed-article:1618983 | pubmed:meshHeading | pubmed-meshheading:1618983-... | lld:pubmed |
| pubmed-article:1618983 | pubmed:meshHeading | pubmed-meshheading:1618983-... | lld:pubmed |
| pubmed-article:1618983 | pubmed:meshHeading | pubmed-meshheading:1618983-... | lld:pubmed |
| pubmed-article:1618983 | pubmed:meshHeading | pubmed-meshheading:1618983-... | lld:pubmed |
| pubmed-article:1618983 | pubmed:meshHeading | pubmed-meshheading:1618983-... | lld:pubmed |
| pubmed-article:1618983 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1618983 | pubmed:articleTitle | Isolation of Der pI, the Dermatophagoides pteronyssinus major mite allergen, from a crude mite culture extract, purification by ion-chromatography, and comparison between the material obtained and a cDNA-coded Der pI. | lld:pubmed |
| pubmed-article:1618983 | pubmed:affiliation | Unité d'Immuno-Allergie, Institut Pasteur, Paris, France. | lld:pubmed |
| pubmed-article:1618983 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1618983 | pubmed:publicationType | Comparative Study | lld:pubmed |
