| pubmed-article:1618855 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1618855 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:1618855 | lifeskim:mentions | umls-concept:C1521970 | lld:lifeskim |
| pubmed-article:1618855 | lifeskim:mentions | umls-concept:C1537665 | lld:lifeskim |
| pubmed-article:1618855 | lifeskim:mentions | umls-concept:C1416956 | lld:lifeskim |
| pubmed-article:1618855 | lifeskim:mentions | umls-concept:C1334043 | lld:lifeskim |
| pubmed-article:1618855 | pubmed:issue | 19 | lld:pubmed |
| pubmed-article:1618855 | pubmed:dateCreated | 1992-8-5 | lld:pubmed |
| pubmed-article:1618855 | pubmed:abstractText | A recently cloned mouse cDNA designated F52 encodes a putative protein with striking sequence similarity to the MARCKS protein, a major cellular substrate for protein kinase C (PKC). Major regions of sequence similarity include the amino-terminal myristoylation consensus sequence and the central calmodulin-binding/PKC phosphorylation site domain. The F52 protein was expressed in Escherichia coli with apparent M(r) 50,000; it was a substrate for PKC and comigrated on two-dimensional electrophoresis with a myristoylated protein whose phosphorylation was stimulated by phorbol 12-myristate 13-acetate in mouse neuroblastoma cells. The F52 protein also was myristoylated in E. coli by co-expression with N-myristoyltransferase. A 24-amino acid peptide derived from the protein's phosphorylation site domain was a good substrate for PKC; like the cognate MARCKS peptide, it was phosphorylated with high affinity (S0.5 = 173 nM) and positive cooperativity (KH = 5.4). The F52 peptide also bound calmodulin with high affinity (Kd = less than 3 nM); this binding could be disrupted by phosphorylation of the peptide with PKC, with a half-time of 8 min. The F52 protein is clearly a member of the MARCKS family as defined by primary sequence; in addition, the two proteins share several key attributes that may be functionally important. | lld:pubmed |
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| pubmed-article:1618855 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1618855 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1618855 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1618855 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:1618855 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1618855 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:1618855 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:1618855 | pubmed:author | pubmed-author:BlackshearP... | lld:pubmed |
| pubmed-article:1618855 | pubmed:author | pubmed-author:JohnsonJ DJD | lld:pubmed |
| pubmed-article:1618855 | pubmed:author | pubmed-author:StumpoD JDJ | lld:pubmed |
| pubmed-article:1618855 | pubmed:author | pubmed-author:HauptD MDM | lld:pubmed |
| pubmed-article:1618855 | pubmed:author | pubmed-author:VergheseG MGM | lld:pubmed |
| pubmed-article:1618855 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1618855 | pubmed:day | 5 | lld:pubmed |
| pubmed-article:1618855 | pubmed:volume | 267 | lld:pubmed |
| pubmed-article:1618855 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1618855 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1618855 | pubmed:pagination | 13540-6 | lld:pubmed |
| pubmed-article:1618855 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:1618855 | pubmed:meshHeading | pubmed-meshheading:1618855-... | lld:pubmed |
| pubmed-article:1618855 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1618855 | pubmed:articleTitle | Characteristics of the F52 protein, a MARCKS homologue. | lld:pubmed |
| pubmed-article:1618855 | pubmed:affiliation | Howard Hughes Medical Institute Laboratories, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710. | lld:pubmed |
| pubmed-article:1618855 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1618855 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1618855 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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