| pubmed-article:16186564 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16186564 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:16186564 | lifeskim:mentions | umls-concept:C0021853 | lld:lifeskim |
| pubmed-article:16186564 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
| pubmed-article:16186564 | lifeskim:mentions | umls-concept:C0037083 | lld:lifeskim |
| pubmed-article:16186564 | lifeskim:mentions | umls-concept:C0250416 | lld:lifeskim |
| pubmed-article:16186564 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:16186564 | lifeskim:mentions | umls-concept:C1823153 | lld:lifeskim |
| pubmed-article:16186564 | lifeskim:mentions | umls-concept:C1710082 | lld:lifeskim |
| pubmed-article:16186564 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:16186564 | lifeskim:mentions | umls-concept:C0162610 | lld:lifeskim |
| pubmed-article:16186564 | lifeskim:mentions | umls-concept:C2349976 | lld:lifeskim |
| pubmed-article:16186564 | lifeskim:mentions | umls-concept:C1552644 | lld:lifeskim |
| pubmed-article:16186564 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:16186564 | pubmed:dateCreated | 2005-9-27 | lld:pubmed |
| pubmed-article:16186564 | pubmed:abstractText | Defecation in the nematode Caenorhabditis elegans is a readily observable ultradian behavioral rhythm that occurs once every 45-50 s and is mediated in part by posterior body wall muscle contraction (pBoc). pBoc is not regulated by neural input but instead is likely controlled by rhythmic Ca(2+) oscillations in the intestinal epithelium. We developed an isolated nematode intestine preparation that allows combined physiological, genetic, and molecular characterization of oscillatory Ca(2+) signaling. Isolated intestines loaded with fluo-4 AM exhibit spontaneous rhythmic Ca(2+) oscillations with a period of approximately 50 s. Oscillations were only detected in the apical cell pole of the intestinal epithelium and occur as a posterior-to-anterior moving intercellular Ca(2+) wave. Loss-of-function mutations in the inositol-1,4,5-trisphosphate (IP(3)) receptor ITR-1 reduce pBoc and Ca(2+) oscillation frequency and intercellular Ca(2+) wave velocity. In contrast, gain-of-function mutations in the IP(3) binding and regulatory domains of ITR-1 have no effect on pBoc or Ca(2+) oscillation frequency but dramatically increase the speed of the intercellular Ca(2+) wave. Systemic RNA interference (RNAi) screening of the six C. elegans phospholipase C (PLC)-encoding genes demonstrated that pBoc and Ca(2+) oscillations require the combined function of PLC-gamma and PLC-beta homologues. Disruption of PLC-gamma and PLC-beta activity by mutation or RNAi induced arrhythmia in pBoc and intestinal Ca(2+) oscillations. The function of the two enzymes is additive. Epistasis analysis suggests that PLC-gamma functions primarily to generate IP(3) that controls ITR-1 activity. In contrast, IP(3) generated by PLC-beta appears to play little or no direct role in ITR-1 regulation. PLC-beta may function instead to control PIP(2) levels and/or G protein signaling events. Our findings provide new insights into intestinal cell Ca(2+) signaling mechanisms and establish C. elegans as a powerful model system for defining the gene networks and molecular mechanisms that underlie the generation and regulation of Ca(2+) oscillations and intercellular Ca(2+) waves in nonexcitable cells. | lld:pubmed |
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| pubmed-article:16186564 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:16186564 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:16186564 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16186564 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:16186564 | pubmed:issn | 0022-1295 | lld:pubmed |
| pubmed-article:16186564 | pubmed:author | pubmed-author:StrangeKevinK | lld:pubmed |
| pubmed-article:16186564 | pubmed:author | pubmed-author:YinXiaoyanX | lld:pubmed |
| pubmed-article:16186564 | pubmed:author | pubmed-author:EstevesAna... | lld:pubmed |
| pubmed-article:16186564 | pubmed:author | pubmed-author:EspeltMaria... | lld:pubmed |
| pubmed-article:16186564 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16186564 | pubmed:volume | 126 | lld:pubmed |
| pubmed-article:16186564 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16186564 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16186564 | pubmed:pagination | 379-92 | lld:pubmed |
| pubmed-article:16186564 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:16186564 | pubmed:meshHeading | pubmed-meshheading:16186564... | lld:pubmed |
| pubmed-article:16186564 | pubmed:meshHeading | pubmed-meshheading:16186564... | lld:pubmed |
| pubmed-article:16186564 | pubmed:meshHeading | pubmed-meshheading:16186564... | lld:pubmed |
| pubmed-article:16186564 | pubmed:meshHeading | pubmed-meshheading:16186564... | lld:pubmed |
