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pubmed-article:1616954pubmed:abstractTextA new controlled-pore glass (CPG) support is described that allows for the direct synthesis of oligonucleotides bearing a 3'-aminohexyl tail. This solid support (AH-CPG) exhibits superior performance as compared to a commercially available 3'-amine CPG. The AH-CPG is prepared from 6-aminohexan-1-ol with a unique protecting group for the amine that also functions as the site of attachment to the CPG. A 3'-amine-tailed oligodeoxynucleotide (ODN) was prepared from this support using standard phosphoramidite coupling and deprotection conditions. The 3'-amine-tailed ODN was subsequently modified with an acridinylpropionic acid tetrafluorophenyl ester. Facile synthesis of the AH-CPG and the stability of the deprotected product makes this functionalized solid support especially useful for preparation of oligonucleotides bearing 3'-amine tails and other modifications.lld:pubmed
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pubmed-article:1616954pubmed:authorpubmed-author:MeyerR BRBJrlld:pubmed
pubmed-article:1616954pubmed:authorpubmed-author:ReedM WMWlld:pubmed
pubmed-article:1616954pubmed:authorpubmed-author:AdamsA DADlld:pubmed
pubmed-article:1616954pubmed:authorpubmed-author:PetrieC RCRlld:pubmed
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pubmed-article:1616954pubmed:pagination85-7lld:pubmed
pubmed-article:1616954pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:1616954pubmed:articleTitleAn improved CPG support for the synthesis of 3'-amine-tailed oligonucleotides.lld:pubmed
pubmed-article:1616954pubmed:affiliationMicroProbe Corporation, Bothell, Washington 98021.lld:pubmed
pubmed-article:1616954pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:1616954pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
pubmed-article:1616954pubmed:publicationTypeResearch Support, U.S. Gov't, Non-P.H.S.lld:pubmed
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