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pubmed-article:16147887pubmed:abstractTextA Pto-like gene (designated as SsPto-2) was isolated from Solanum surattense by using genomic walker technology which encoded a cytoplasmically localized serine-threonine protein kinase. Analysis of the 2365 bp segment revealed a gene including a 905 bp 5' flanking region, a 924 bp open reading frame (ORF) and a 536 bp 3' flanking region. The deduced amino acid sequence of the SsPto-2 gene shared high homology with other known Ptos. The deduced SsPto-2 protein contained no signal peptide with a calculated molecular weight of 34.61 kDa. The analysis of SsPto-2 promoter region and terminator region was also presented. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed that SsPto-2 transcripts were up-regulated by defense-related factors such as gibberellic acid (GA(3)), salicylic acid (SA) and down-regulated by darkness. The cloning of the SsPto-2 gene will allow us to further study its potential role in disease resistance.lld:pubmed
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pubmed-article:16147887pubmed:authorpubmed-author:LinJuanJlld:pubmed
pubmed-article:16147887pubmed:authorpubmed-author:LiuXiaojunXlld:pubmed
pubmed-article:16147887pubmed:authorpubmed-author:SunXiaofenXlld:pubmed
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pubmed-article:16147887pubmed:authorpubmed-author:WangXinglongXlld:pubmed
pubmed-article:16147887pubmed:authorpubmed-author:HuangBeibeiBlld:pubmed
pubmed-article:16147887pubmed:authorpubmed-author:ZengHainianHlld:pubmed
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pubmed-article:16147887pubmed:pagination277-87lld:pubmed
pubmed-article:16147887pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:16147887pubmed:year2005lld:pubmed
pubmed-article:16147887pubmed:articleTitleGenomic cloning and characterization of a Pto-like gene SsPto-2 from Solanum surattense.lld:pubmed
pubmed-article:16147887pubmed:affiliationState Key Laboratory of Genetic Engineering, School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai, 200433, P. R. China.lld:pubmed
pubmed-article:16147887pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:16147887pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed