| pubmed-article:16139362 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16139362 | lifeskim:mentions | umls-concept:C0220806 | lld:lifeskim |
| pubmed-article:16139362 | lifeskim:mentions | umls-concept:C0999009 | lld:lifeskim |
| pubmed-article:16139362 | lifeskim:mentions | umls-concept:C0040132 | lld:lifeskim |
| pubmed-article:16139362 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:16139362 | lifeskim:mentions | umls-concept:C0025558 | lld:lifeskim |
| pubmed-article:16139362 | lifeskim:mentions | umls-concept:C1527148 | lld:lifeskim |
| pubmed-article:16139362 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:16139362 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:16139362 | pubmed:dateCreated | 2006-6-9 | lld:pubmed |
| pubmed-article:16139362 | pubmed:abstractText | The West African clawed frog (Silurana tropicalis), which resembles the South African clawed frog (Xenopus laevis), but is somewhat smaller, has a diploid genome and a shorter generation time. Therefore, S. tropicalis has the potential for use as a new model in ecotoxicology. We demonstrated a S. tropicalis metamorphosis assay based on Xenopus Metamorphosis Assay (XEMA) using 1 microg/L thyroxine (T4) and 75 mg/L propylthiouracil (PTU). Tadpoles at developmental stages 48-50 were exposed to chemicals for 28 days and total body length, developmental stage, and hind limb length were recorded every 7 days. Significant differences in developmental stage and total body length were found for both T4 and PTU after 7-day exposure, which were similar to the results of the XEMA ring-test using the same chemicals. Moreover, in the present study, we measured hind limb length as a new endpoint of thyroid axis. Significant differences in the hind limb length were encountered in both T4 and PTU treatments after 7 days of exposure. These results suggest that S. tropicalis can be used in a XEMA-like protocol to detect agonist and antagonist effects of chemicals on the thyroid system. Hind limb length is also a suitable endpoint in such protocols. A new test protocol detecting both thyroid disruption and reproductive effects of chemicals using S. tropicalis should be established in the near future. | lld:pubmed |
| pubmed-article:16139362 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16139362 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16139362 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16139362 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16139362 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16139362 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16139362 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16139362 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16139362 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:16139362 | pubmed:issn | 0147-6513 | lld:pubmed |
| pubmed-article:16139362 | pubmed:author | pubmed-author:IguchiTaisenT | lld:pubmed |
| pubmed-article:16139362 | pubmed:author | pubmed-author:KashiwagiKeik... | lld:pubmed |
| pubmed-article:16139362 | pubmed:author | pubmed-author:FujiiTakaakiT | lld:pubmed |
| pubmed-article:16139362 | pubmed:author | pubmed-author:HanadaHidekiH | lld:pubmed |
| pubmed-article:16139362 | pubmed:author | pubmed-author:UrushitaniHir... | lld:pubmed |
| pubmed-article:16139362 | pubmed:author | pubmed-author:KashiwagiAkih... | lld:pubmed |
| pubmed-article:16139362 | pubmed:author | pubmed-author:MitsuiNaokoN | lld:pubmed |
| pubmed-article:16139362 | pubmed:author | pubmed-author:TooiOsamuO | lld:pubmed |
| pubmed-article:16139362 | pubmed:author | pubmed-author:OkaTomohiroT | lld:pubmed |
| pubmed-article:16139362 | pubmed:author | pubmed-author:SantoNoriakiN | lld:pubmed |
| pubmed-article:16139362 | pubmed:author | pubmed-author:MiyaharaMakiM | lld:pubmed |
| pubmed-article:16139362 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16139362 | pubmed:volume | 64 | lld:pubmed |
| pubmed-article:16139362 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16139362 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16139362 | pubmed:pagination | 281-7 | lld:pubmed |
| pubmed-article:16139362 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:16139362 | pubmed:meshHeading | pubmed-meshheading:16139362... | lld:pubmed |
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| pubmed-article:16139362 | pubmed:meshHeading | pubmed-meshheading:16139362... | lld:pubmed |
| pubmed-article:16139362 | pubmed:meshHeading | pubmed-meshheading:16139362... | lld:pubmed |
| pubmed-article:16139362 | pubmed:meshHeading | pubmed-meshheading:16139362... | lld:pubmed |
| pubmed-article:16139362 | pubmed:meshHeading | pubmed-meshheading:16139362... | lld:pubmed |
| pubmed-article:16139362 | pubmed:meshHeading | pubmed-meshheading:16139362... | lld:pubmed |
| pubmed-article:16139362 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16139362 | pubmed:articleTitle | Development of metamorphosis assay using Silurana tropicalis for the detection of thyroid system-disrupting chemicals. | lld:pubmed |
| pubmed-article:16139362 | pubmed:affiliation | Biotechnology Research Laboratory, Towa Kagaku Co., Ltd., 3-13-26 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan. | lld:pubmed |
| pubmed-article:16139362 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16139362 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:16139362 | pubmed:publicationType | Validation Studies | lld:pubmed |
