| pubmed-article:16135545 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16135545 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:16135545 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:16135545 | lifeskim:mentions | umls-concept:C0036536 | lld:lifeskim |
| pubmed-article:16135545 | lifeskim:mentions | umls-concept:C0036537 | lld:lifeskim |
| pubmed-article:16135545 | lifeskim:mentions | umls-concept:C0022378 | lld:lifeskim |
| pubmed-article:16135545 | lifeskim:mentions | umls-concept:C0596019 | lld:lifeskim |
| pubmed-article:16135545 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:16135545 | pubmed:dateCreated | 2005-12-12 | lld:pubmed |
| pubmed-article:16135545 | pubmed:abstractText | We investigated the effects of 5,6-dichloro-1-ethyl-1,3-dihydro-2H-benzimidazol-2-one(DCEBIO) on the Cl- secretory response of the mouse jejunum using the Ussing short-circuit current (Isc) technique. DCEBIO stimulated a concentration-dependent, sustained increase in Isc (EC50 41 +/- 1 microM). Pretreating tissues with 0.25 microM forskolin reduced the concentration-dependent increase in Isc by DCEBIO and increased the EC50 (53 +/- 5 microM). Bumetanide blocked (82 +/- 5%) the DCEBIO-stimulated Isc consistent with Cl- secretion. DCEBIO was a more potent stimulator of Cl- secretion than its parent molecule, 1-ethyl-2-benzimidazolinone. Glibenclamide or NPPB reduced the DCEBIO-stimulated Isc by >80% indicating the participation of CFTR in the DCEBIO-stimulated Isc response. Clotrimazole reduced DCEBIO-stimulated Isc by 67 +/- 15%, suggesting the participation of the intermediate conductance Ca2+-activated K+ channel (IKCa) in the DCEBIO-activated Isc response. In the presence of maximum forskolin (10 microM), the DCEBIO response was reduced and biphasic, reaching a peak response of the change in Isc of 43 +/- 5 microA/cm2 and then falling to a steady-state response of 17 +/- 10 microA/cm2 compared with DCEBIO control tissues (61 +/- 6 microA/cm2). The forskolin-stimulated Isc in the presence of DCEBIO was reduced compared with forskolin control tissues. Similar results were observed with DCEBIO and 8-BrcAMP where adenylate cyclase was bypassed. H89, a PKA inhibitor, reduced the DCEBIO-activated Isc, providing evidence that DCEBIO increased Cl- secretion via a cAMP/PKA-dependent manner. These data suggest that DCEBIO stimulates Cl- secretion of the mouse jejunum and that DCEBIO targets components of the Cl- secretory mechanism. | lld:pubmed |
| pubmed-article:16135545 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16135545 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16135545 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16135545 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16135545 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16135545 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16135545 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16135545 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16135545 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16135545 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16135545 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16135545 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16135545 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16135545 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:16135545 | pubmed:issn | 0363-6143 | lld:pubmed |
| pubmed-article:16135545 | pubmed:author | pubmed-author:HamiltonKirk... | lld:pubmed |
| pubmed-article:16135545 | pubmed:author | pubmed-author:KiesslingMatt... | lld:pubmed |
| pubmed-article:16135545 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16135545 | pubmed:volume | 290 | lld:pubmed |
| pubmed-article:16135545 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16135545 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16135545 | pubmed:pagination | C152-64 | lld:pubmed |
| pubmed-article:16135545 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:16135545 | pubmed:meshHeading | pubmed-meshheading:16135545... | lld:pubmed |
| pubmed-article:16135545 | pubmed:meshHeading | pubmed-meshheading:16135545... | lld:pubmed |
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| pubmed-article:16135545 | pubmed:meshHeading | pubmed-meshheading:16135545... | lld:pubmed |
| pubmed-article:16135545 | pubmed:meshHeading | pubmed-meshheading:16135545... | lld:pubmed |
| pubmed-article:16135545 | pubmed:meshHeading | pubmed-meshheading:16135545... | lld:pubmed |
| pubmed-article:16135545 | pubmed:meshHeading | pubmed-meshheading:16135545... | lld:pubmed |
| pubmed-article:16135545 | pubmed:meshHeading | pubmed-meshheading:16135545... | lld:pubmed |
| pubmed-article:16135545 | pubmed:meshHeading | pubmed-meshheading:16135545... | lld:pubmed |
| pubmed-article:16135545 | pubmed:meshHeading | pubmed-meshheading:16135545... | lld:pubmed |
| pubmed-article:16135545 | pubmed:meshHeading | pubmed-meshheading:16135545... | lld:pubmed |
| pubmed-article:16135545 | pubmed:meshHeading | pubmed-meshheading:16135545... | lld:pubmed |
| pubmed-article:16135545 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16135545 | pubmed:articleTitle | DCEBIO stimulates Cl- secretion in the mouse jejunum. | lld:pubmed |
| pubmed-article:16135545 | pubmed:affiliation | Dept. of Physiology, School of Medical Sciences, Univ. of Otago, PO Box 913, Dunedin, New Zealand. kirk.hamilton@stonebow.otago.ac.nz | lld:pubmed |
| pubmed-article:16135545 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16135545 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16135545 | lld:pubmed |
