| pubmed-article:16133333 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16133333 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:16133333 | lifeskim:mentions | umls-concept:C0016219 | lld:lifeskim |
| pubmed-article:16133333 | lifeskim:mentions | umls-concept:C0060444 | lld:lifeskim |
| pubmed-article:16133333 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:16133333 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:16133333 | pubmed:dateCreated | 2006-5-31 | lld:pubmed |
| pubmed-article:16133333 | pubmed:abstractText | (2S)-Flavanones (naringenin and pinocembrin) are key intermediates in the flavonoid biosynthetic pathway in plants. Recombinant Escherichia coli cells containing four genes for a phenylalanine ammonia-lyase, cinnamate/coumarate:CoA ligase, chalcone synthase, and chalcone isomerase, in addition to the acetyl-CoA carboxylase, have been established for efficient production of (2S)-naringenin from tyrosine and (2S)-pinocembrin from phenylalanine. Further introduction of the flavone synthase I gene from Petroselinum crispum under the control of the T7 promoter and the synthetic ribosome-binding sequence in pACYCDuet-1 caused the E. coli cells to produce flavones: apigenin (13 mg/l) from tyrosine and chrysin (9.4 mg/l) from phenylalanine. Introduction into the E. coli cells of the flavanone 3beta-hydroxylase and flavonol synthase genes from the plant Citrus species led to production of flavonols: kaempferol (15.1 mg/l) from tyrosine and galangin (1.1 mg/l) from phenylalanine. The combinatorial biosynthesis of the flavones and flavonols in E. coli is promising for the construction of a library of various flavonoid compounds and un-natural flavonoids in bacteria. | lld:pubmed |
| pubmed-article:16133333 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16133333 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16133333 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16133333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16133333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16133333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16133333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16133333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16133333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16133333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16133333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16133333 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16133333 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:16133333 | pubmed:issn | 0175-7598 | lld:pubmed |
| pubmed-article:16133333 | pubmed:author | pubmed-author:OhnishiYasuoY | lld:pubmed |
| pubmed-article:16133333 | pubmed:author | pubmed-author:HorinouchiSue... | lld:pubmed |
| pubmed-article:16133333 | pubmed:author | pubmed-author:FunaNobutakaN | lld:pubmed |
| pubmed-article:16133333 | pubmed:author | pubmed-author:MoriguchiTaka... | lld:pubmed |
| pubmed-article:16133333 | pubmed:author | pubmed-author:MartensStefan... | lld:pubmed |
| pubmed-article:16133333 | pubmed:author | pubmed-author:MiyahisaIkuoI | lld:pubmed |
| pubmed-article:16133333 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16133333 | pubmed:volume | 71 | lld:pubmed |
| pubmed-article:16133333 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16133333 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16133333 | pubmed:pagination | 53-8 | lld:pubmed |
| pubmed-article:16133333 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:16133333 | pubmed:meshHeading | pubmed-meshheading:16133333... | lld:pubmed |
| pubmed-article:16133333 | pubmed:meshHeading | pubmed-meshheading:16133333... | lld:pubmed |
| pubmed-article:16133333 | pubmed:meshHeading | pubmed-meshheading:16133333... | lld:pubmed |
| pubmed-article:16133333 | pubmed:meshHeading | pubmed-meshheading:16133333... | lld:pubmed |
| pubmed-article:16133333 | pubmed:meshHeading | pubmed-meshheading:16133333... | lld:pubmed |
| pubmed-article:16133333 | pubmed:meshHeading | pubmed-meshheading:16133333... | lld:pubmed |
| pubmed-article:16133333 | pubmed:meshHeading | pubmed-meshheading:16133333... | lld:pubmed |
| pubmed-article:16133333 | pubmed:meshHeading | pubmed-meshheading:16133333... | lld:pubmed |
| pubmed-article:16133333 | pubmed:meshHeading | pubmed-meshheading:16133333... | lld:pubmed |
| pubmed-article:16133333 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16133333 | pubmed:articleTitle | Combinatorial biosynthesis of flavones and flavonols in Escherichia coli. | lld:pubmed |
| pubmed-article:16133333 | pubmed:affiliation | Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, 113-8657, Japan. | lld:pubmed |
| pubmed-article:16133333 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16133333 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16133333 | lld:pubmed |
