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pubmed-article:16129513pubmed:abstractTextTwo novel inflammatory peptides were isolated from the venom of the social wasp Polybia paulista. They had their molecular masses determined by ESI-MS and their primary sequences were elucidated by Edman degradation chemistry as: Polybia-MPI: I D W K K L L D A A K Q I L-NH2 (1654.09 Da), Polybia-CP: I L G T I L G L L K S L-NH2 (1239.73 Da). Both peptides were functionally characterized by using Wistar rat cells. Polybia-MPI is a mast cell lytic peptide, which causes no hemolysis to rat erythrocytes and presents chemotaxis for polymorphonucleated leukocytes (PMNL) and with potent antimicrobial action both against Gram-positive and Gram-negative bacteria. Polybia-CP was characterized as a chemotactic peptide for PMNL cells, presenting antimicrobial action against Gram-positive bacteria, but causing no hemolysis to rat erythrocytes and no mast cell degranulation activity at physiological concentrations.lld:pubmed
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pubmed-article:16129513pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:16129513pubmed:articleTitleStructural and functional characterization of two novel peptide toxins isolated from the venom of the social wasp Polybia paulista.lld:pubmed
pubmed-article:16129513pubmed:affiliationCEIS, Department of Biology/IBRC-UNESP (CAT-CEPID/FAPESP), Institute of Immunological Investigations (Millennium Institute-MCT/CNPq), Rio Claro, SP 13506-900, Brazil.lld:pubmed
pubmed-article:16129513pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:16129513pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed