| pubmed-article:16125053 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16125053 | lifeskim:mentions | umls-concept:C1704336 | lld:lifeskim |
| pubmed-article:16125053 | lifeskim:mentions | umls-concept:C0162638 | lld:lifeskim |
| pubmed-article:16125053 | lifeskim:mentions | umls-concept:C0079904 | lld:lifeskim |
| pubmed-article:16125053 | lifeskim:mentions | umls-concept:C0255813 | lld:lifeskim |
| pubmed-article:16125053 | lifeskim:mentions | umls-concept:C0013081 | lld:lifeskim |
| pubmed-article:16125053 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:16125053 | lifeskim:mentions | umls-concept:C0205217 | lld:lifeskim |
| pubmed-article:16125053 | lifeskim:mentions | umls-concept:C1292733 | lld:lifeskim |
| pubmed-article:16125053 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:16125053 | pubmed:dateCreated | 2005-8-29 | lld:pubmed |
| pubmed-article:16125053 | pubmed:abstractText | Wasting of skeletal muscle (cachexia) is associated with a variety of chronic or inflammatory disorders and has long been recognized as a poor prognostic sign. It is currently accepted that the cytokine tumor necrosis factor alpha (TNF-alpha; cachectin) plays a key role in the development of this condition. TNF-alpha-induced apoptotic cell death represents a potential mechanism by which muscle wasting can occur. Evidence has accumulated that the cytokine interferon gamma (IFN-gamma) may act as a modulator of TNF-alpha signalling. Thus, the present study was designed to elucidate if TNF-alpha can directly induce apoptosis in differentiated myotubes, to assess the potential anti-apoptotic properties of IFN-gamma and to get insight into the signalling pathways implicated in the modulatory effects of IFN-gamma. Myoblasts of the murine cell line C2C12 were allowed to differentiate in a low serum containing media and myogenesis assessed by muscle specific protein expression. Non-proliferating, polynucleated, fully differentiated myotubes were obtained after seven days in differentiation media. Exposure of C2C12 myotubes to TNF-alpha for 48 h induced apoptosis characterized by enhanced caspase-3 activity, which resulted in poly(ADP-ribose) polymerase (PARP) cleavage and increased histone-associated-DNA fragmentation. These effects were fully reverted in the presence of IFN-gamma. This cytokine induced down-regulation of the subtype 2 of TNF-alpha receptors (TNF-R2), enhanced TNF-alpha-induced NF-kappaB translocation to the nucleus and binding to DNA and increased the immunoreactivity of the protein c-IAP1, a member of the inhibitor of apoptosis (IAP) gene family whose synthesis is stimulated by NF-kappaB at the transcriptional level. Together, these results demonstrate that TNF-alpha directly induces apoptosis in differentiated myotubes and suggest that the cytokine IFN-gamma, might represent a new immunoadjuvant therapeutic tool for managing cachexia. | lld:pubmed |
| pubmed-article:16125053 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16125053 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16125053 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16125053 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16125053 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16125053 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16125053 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16125053 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16125053 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16125053 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16125053 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16125053 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16125053 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16125053 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16125053 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:16125053 | pubmed:issn | 0898-6568 | lld:pubmed |
| pubmed-article:16125053 | pubmed:author | pubmed-author:BusquetsXavie... | lld:pubmed |
| pubmed-article:16125053 | pubmed:author | pubmed-author:LladóJeròniaJ | lld:pubmed |
| pubmed-article:16125053 | pubmed:author | pubmed-author:TolosaLaiaL | lld:pubmed |
| pubmed-article:16125053 | pubmed:author | pubmed-author:MorláMontseM | lld:pubmed |
| pubmed-article:16125053 | pubmed:author | pubmed-author:IglesiasAmand... | lld:pubmed |
| pubmed-article:16125053 | pubmed:author | pubmed-author:OlmosGabrielG | lld:pubmed |
| pubmed-article:16125053 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16125053 | pubmed:volume | 17 | lld:pubmed |
| pubmed-article:16125053 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16125053 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16125053 | pubmed:pagination | 1333-42 | lld:pubmed |
| pubmed-article:16125053 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:16125053 | pubmed:meshHeading | pubmed-meshheading:16125053... | lld:pubmed |
| pubmed-article:16125053 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:16125053 | pubmed:articleTitle | IFN-gamma prevents TNF-alpha-induced apoptosis in C2C12 myotubes through down-regulation of TNF-R2 and increased NF-kappaB activity. | lld:pubmed |
| pubmed-article:16125053 | pubmed:affiliation | Institut Universitari d'Investigacions en Ciències de la Salut/Departament de Biologia, Universitat de les Illes Balears, E-07122 Palma de Mallorca, Spain. | lld:pubmed |
| pubmed-article:16125053 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16125053 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16125053 | lld:pubmed |
