| pubmed-article:16061933 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16061933 | lifeskim:mentions | umls-concept:C0439849 | lld:lifeskim |
| pubmed-article:16061933 | lifeskim:mentions | umls-concept:C0205145 | lld:lifeskim |
| pubmed-article:16061933 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:16061933 | lifeskim:mentions | umls-concept:C0547040 | lld:lifeskim |
| pubmed-article:16061933 | lifeskim:mentions | umls-concept:C0445223 | lld:lifeskim |
| pubmed-article:16061933 | lifeskim:mentions | umls-concept:C1881217 | lld:lifeskim |
| pubmed-article:16061933 | lifeskim:mentions | umls-concept:C1552599 | lld:lifeskim |
| pubmed-article:16061933 | lifeskim:mentions | umls-concept:C1704787 | lld:lifeskim |
| pubmed-article:16061933 | lifeskim:mentions | umls-concept:C1515655 | lld:lifeskim |
| pubmed-article:16061933 | lifeskim:mentions | umls-concept:C0442335 | lld:lifeskim |
| pubmed-article:16061933 | lifeskim:mentions | umls-concept:C0444930 | lld:lifeskim |
| pubmed-article:16061933 | pubmed:issue | 13 | lld:pubmed |
| pubmed-article:16061933 | pubmed:dateCreated | 2005-8-2 | lld:pubmed |
| pubmed-article:16061933 | pubmed:abstractText | Contrary to several earlier reports, we find that cross-recombination between wild-type and the mutant loxP511 sites is <0.5% of that between two wild-type sites if Cre protein is expressed by phage P1 during an infection. The finding enabled us to develop a procedure to truncate DNA progressively from both ends of large genomic inserts flanked by these two loxP sites in pBACe3.6 and related vectors with transposons carrying either a wild-type or a loxP511 sequence. Newly constructed loxP511 transposons contained either a kanamycin resistance gene or no marker. Insert DNA ends in deletions were sequenced with primers unique to each transposon-end remaining after the respective recombination. End-sequencing 223 deletions confirmed that the low level of cross-recombination, observed between those sites during the P1 transductions, does not complicate the procedure: truncations from the unintended end of genomic inserts did not occur. Multiple BACs pooled together could also be processed in a single tube to make end-deletions. This deletion technology, utilizing the very minimal cross-recombination between the mutant and wild-type loxP sites of most BAC clones in the public domain and a heterologous one inserted as a transposon, should facilitate functionally mapping long-range gene regulatory sequences and help to isolate genes with defined functional boundaries in numerous projects including those of therapeutic interest. | lld:pubmed |
| pubmed-article:16061933 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16061933 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16061933 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16061933 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16061933 | pubmed:issn | 1362-4962 | lld:pubmed |
| pubmed-article:16061933 | pubmed:author | pubmed-author:ChatterjeePra... | lld:pubmed |
| pubmed-article:16061933 | pubmed:author | pubmed-author:SrivastavaDee... | lld:pubmed |
| pubmed-article:16061933 | pubmed:author | pubmed-author:ShakesLeighcr... | lld:pubmed |
| pubmed-article:16061933 | pubmed:author | pubmed-author:GarlandDougla... | lld:pubmed |
| pubmed-article:16061933 | pubmed:author | pubmed-author:HarewoodKen... | lld:pubmed |
| pubmed-article:16061933 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:16061933 | pubmed:volume | 33 | lld:pubmed |
| pubmed-article:16061933 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16061933 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16061933 | pubmed:pagination | e118 | lld:pubmed |
| pubmed-article:16061933 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:16061933 | pubmed:meshHeading | pubmed-meshheading:16061933... | lld:pubmed |
| pubmed-article:16061933 | pubmed:meshHeading | pubmed-meshheading:16061933... | lld:pubmed |
| pubmed-article:16061933 | pubmed:meshHeading | pubmed-meshheading:16061933... | lld:pubmed |
| pubmed-article:16061933 | pubmed:meshHeading | pubmed-meshheading:16061933... | lld:pubmed |
| pubmed-article:16061933 | pubmed:meshHeading | pubmed-meshheading:16061933... | lld:pubmed |
| pubmed-article:16061933 | pubmed:meshHeading | pubmed-meshheading:16061933... | lld:pubmed |
| pubmed-article:16061933 | pubmed:meshHeading | pubmed-meshheading:16061933... | lld:pubmed |
| pubmed-article:16061933 | pubmed:meshHeading | pubmed-meshheading:16061933... | lld:pubmed |
| pubmed-article:16061933 | pubmed:meshHeading | pubmed-meshheading:16061933... | lld:pubmed |
| pubmed-article:16061933 | pubmed:meshHeading | pubmed-meshheading:16061933... | lld:pubmed |
| pubmed-article:16061933 | pubmed:meshHeading | pubmed-meshheading:16061933... | lld:pubmed |
| pubmed-article:16061933 | pubmed:meshHeading | pubmed-meshheading:16061933... | lld:pubmed |
| pubmed-article:16061933 | pubmed:meshHeading | pubmed-meshheading:16061933... | lld:pubmed |
| pubmed-article:16061933 | pubmed:meshHeading | pubmed-meshheading:16061933... | lld:pubmed |
| pubmed-article:16061933 | pubmed:meshHeading | pubmed-meshheading:16061933... | lld:pubmed |
| pubmed-article:16061933 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:16061933 | pubmed:articleTitle | Minimal cross-recombination between wild-type and loxP511 sites in vivo facilitates truncating both ends of large DNA inserts in pBACe3.6 and related vectors. | lld:pubmed |
