| pubmed-article:16051808 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16051808 | lifeskim:mentions | umls-concept:C1041448 | lld:lifeskim |
| pubmed-article:16051808 | lifeskim:mentions | umls-concept:C1314972 | lld:lifeskim |
| pubmed-article:16051808 | lifeskim:mentions | umls-concept:C1622204 | lld:lifeskim |
| pubmed-article:16051808 | lifeskim:mentions | umls-concept:C0018909 | lld:lifeskim |
| pubmed-article:16051808 | lifeskim:mentions | umls-concept:C0162768 | lld:lifeskim |
| pubmed-article:16051808 | lifeskim:mentions | umls-concept:C0237497 | lld:lifeskim |
| pubmed-article:16051808 | lifeskim:mentions | umls-concept:C0242732 | lld:lifeskim |
| pubmed-article:16051808 | lifeskim:mentions | umls-concept:C0599894 | lld:lifeskim |
| pubmed-article:16051808 | lifeskim:mentions | umls-concept:C0185023 | lld:lifeskim |
| pubmed-article:16051808 | lifeskim:mentions | umls-concept:C0443288 | lld:lifeskim |
| pubmed-article:16051808 | lifeskim:mentions | umls-concept:C1947904 | lld:lifeskim |
| pubmed-article:16051808 | lifeskim:mentions | umls-concept:C0037791 | lld:lifeskim |
| pubmed-article:16051808 | lifeskim:mentions | umls-concept:C1999228 | lld:lifeskim |
| pubmed-article:16051808 | lifeskim:mentions | umls-concept:C2825781 | lld:lifeskim |
| pubmed-article:16051808 | pubmed:issue | 16 | lld:pubmed |
| pubmed-article:16051808 | pubmed:dateCreated | 2005-7-29 | lld:pubmed |
| pubmed-article:16051808 | pubmed:abstractText | To engineer a targeting envelope for gene and oncolytic vector delivery, we characterized and modified the envelope proteins of Tupaia paramyxovirus (TPMV), a relative of the morbilli- and henipaviruses that neither infects humans nor has cross-reactive relatives that infect humans. We completed the TPMV genomic sequence and noted that the predicted fusion (F) protein cleavage-activation site is not preceded by a canonical furin cleavage sequence. Coexpression of the TPMV F and hemagglutinin (H) proteins induced fusion of Tupaia baby fibroblasts but not of human cells, a finding consistent with the restricted TPMV host range. To identify the factors restricting fusion of non-Tupaia cells, we initially analyzed F protein cleavage. Even without an oligo- or monobasic protease cleavage sequence, TPMV F was cleaved in F1 and F2 subunits in human cells. Edman degradation of the F1 subunit yielded the sequence IFWGAIIA, placing the conserved phenylalanine in position 2, a novelty for paramyxoviruses but not the cause of fusion restriction. We then verified whether the lack of a TPMV H receptor limits fusion. Toward this end, we displayed a single-chain antibody (scFv) specific for the designated receptor human carcinoembryonic antigen on the TPMV H ectodomain. The H-scFv hybrid protein coexpressed with TPMV F mediated fusion of cells expressing the designated receptor, proving that the lack of a receptor limits fusion and that TPMV H can be retargeted. Targeting competence and the absence of antibodies in humans define the TPMV envelope as a module to be adapted for ferrying ribonucleocapsids of oncolytic viruses and gene delivery vectors. | lld:pubmed |
| pubmed-article:16051808 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16051808 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16051808 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16051808 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16051808 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:16051808 | pubmed:issn | 0022-538X | lld:pubmed |
| pubmed-article:16051808 | pubmed:author | pubmed-author:CattaneoRober... | lld:pubmed |
| pubmed-article:16051808 | pubmed:author | pubmed-author:von... | lld:pubmed |
| pubmed-article:16051808 | pubmed:author | pubmed-author:DaraiGholamre... | lld:pubmed |
| pubmed-article:16051808 | pubmed:author | pubmed-author:SpringfeldChr... | lld:pubmed |
| pubmed-article:16051808 | pubmed:author | pubmed-author:TidonaChristi... | lld:pubmed |
| pubmed-article:16051808 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16051808 | pubmed:volume | 79 | lld:pubmed |
| pubmed-article:16051808 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16051808 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16051808 | pubmed:pagination | 10155-63 | lld:pubmed |
| pubmed-article:16051808 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:16051808 | pubmed:meshHeading | pubmed-meshheading:16051808... | lld:pubmed |
