| pubmed-article:16044965 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16044965 | lifeskim:mentions | umls-concept:C0699791 | lld:lifeskim |
| pubmed-article:16044965 | lifeskim:mentions | umls-concept:C0029298 | lld:lifeskim |
| pubmed-article:16044965 | lifeskim:mentions | umls-concept:C0014441 | lld:lifeskim |
| pubmed-article:16044965 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:16044965 | pubmed:dateCreated | 2005-7-27 | lld:pubmed |
| pubmed-article:16044965 | pubmed:abstractText | A number of enzymes have been shown to be involved in the process of activation and/or degradation of 5-fluorouracil, and they are potential candidates for predicting factors of chemosensitivity to 5-fluorouracil. Among them, orotate phosphoribosyltransferase (OPRT EC 2.4.2.10) is a key enzyme related to the first-step activation process of 5-fluorouracil and therefore it has been shown to be an important enzyme for the prediction of sensitivity to 5-fluorouracil and its related derivatives. We developed a new enzyme-linked immunosorbent assay (ELISA) system to accurately assess intratumoral activity of orotate phosphoribosyltransferase. A new sandwich ELISA system was established using anti-OPRT polyclonal antibodies obtained from the rabbit immunized with the recombinant human peptides of the OPRT molecule. OPRT levels were measured in 8 human cancer xenografts transplanted in nude mice and 58 gastric cancer tissues using both a newly established ELISA and a conventional enzyme assay using radiolabeled 5-fluorouracil as a substrate. OPRT levels in 8 human cancer xenografts measured by this ELISA were significantly correlated with the OPRT enzyme activities (r2=0.782). Furthermore, OPRT activities measured in 58 gastric cancer tissues by enzyme assay were significantly correlated with those measured by the newly-established ELISA (r2=0.664). The ELISA system developed for the measurement of OPRT required a minimal amount of carcinoma tissue samples, which could be an easy-of-use assay system to predict sensitivity to 5-fluorouracil in gastric carcinoma. These results suggest that this newly-developed sandwich ELISA system for the quantification of OPRT is technically simple, feasible, and may be a useful tool to predict sensitivity to fluoropyrimidine-based anticancer chemotherapy in patients with gastric carcinoma and other cancers. | lld:pubmed |
| pubmed-article:16044965 | pubmed:language | jpn | lld:pubmed |
| pubmed-article:16044965 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16044965 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16044965 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16044965 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16044965 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16044965 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:16044965 | pubmed:issn | 0385-0684 | lld:pubmed |
| pubmed-article:16044965 | pubmed:author | pubmed-author:FukushimaMasa... | lld:pubmed |
| pubmed-article:16044965 | pubmed:author | pubmed-author:SugimotoYoshi... | lld:pubmed |
| pubmed-article:16044965 | pubmed:author | pubmed-author:TanakaHideyuk... | lld:pubmed |
| pubmed-article:16044965 | pubmed:author | pubmed-author:NakamuraYasuk... | lld:pubmed |
| pubmed-article:16044965 | pubmed:author | pubmed-author:SakuraiYoichi... | lld:pubmed |
| pubmed-article:16044965 | pubmed:author | pubmed-author:OchiaiMasahir... | lld:pubmed |
| pubmed-article:16044965 | pubmed:author | pubmed-author:UyamaIchiroI | lld:pubmed |
| pubmed-article:16044965 | pubmed:author | pubmed-author:KomoriYoshiyu... | lld:pubmed |
| pubmed-article:16044965 | pubmed:author | pubmed-author:MasuiToshihik... | lld:pubmed |
| pubmed-article:16044965 | pubmed:author | pubmed-author:SakamotoKazuk... | lld:pubmed |
| pubmed-article:16044965 | pubmed:author | pubmed-author:YoshidaIkuoI | lld:pubmed |
| pubmed-article:16044965 | pubmed:author | pubmed-author:TonomuraShuhe... | lld:pubmed |
| pubmed-article:16044965 | pubmed:author | pubmed-author:ShojiMitsutak... | lld:pubmed |
| pubmed-article:16044965 | pubmed:author | pubmed-author:OkaToshinoriT | lld:pubmed |
| pubmed-article:16044965 | pubmed:author | pubmed-author:InabaKazukiK | lld:pubmed |
| pubmed-article:16044965 | pubmed:author | pubmed-author:MatsuuraShiro... | lld:pubmed |
| pubmed-article:16044965 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16044965 | pubmed:volume | 32 | lld:pubmed |
| pubmed-article:16044965 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16044965 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16044965 | pubmed:pagination | 1017-22 | lld:pubmed |
| pubmed-article:16044965 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:16044965 | pubmed:meshHeading | pubmed-meshheading:16044965... | lld:pubmed |
| pubmed-article:16044965 | pubmed:meshHeading | pubmed-meshheading:16044965... | lld:pubmed |
| pubmed-article:16044965 | pubmed:meshHeading | pubmed-meshheading:16044965... | lld:pubmed |
| pubmed-article:16044965 | pubmed:meshHeading | pubmed-meshheading:16044965... | lld:pubmed |
| pubmed-article:16044965 | pubmed:meshHeading | pubmed-meshheading:16044965... | lld:pubmed |
| pubmed-article:16044965 | pubmed:meshHeading | pubmed-meshheading:16044965... | lld:pubmed |
| pubmed-article:16044965 | pubmed:meshHeading | pubmed-meshheading:16044965... | lld:pubmed |
| pubmed-article:16044965 | pubmed:meshHeading | pubmed-meshheading:16044965... | lld:pubmed |
| pubmed-article:16044965 | pubmed:meshHeading | pubmed-meshheading:16044965... | lld:pubmed |
| pubmed-article:16044965 | pubmed:meshHeading | pubmed-meshheading:16044965... | lld:pubmed |
| pubmed-article:16044965 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:16044965 | pubmed:articleTitle | [Establishment of enzyme-linked immunosorbent assay for quantification of orotate phosphoribosyltransferase in gastric carcinoma]. | lld:pubmed |
| pubmed-article:16044965 | pubmed:affiliation | Dept. of Surgery, Fujita Health University School of Medicine. | lld:pubmed |
| pubmed-article:16044965 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16044965 | pubmed:publicationType | English Abstract | lld:pubmed |
