| pubmed-article:16030218 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16030218 | lifeskim:mentions | umls-concept:C0033809 | lld:lifeskim |
| pubmed-article:16030218 | lifeskim:mentions | umls-concept:C0020275 | lld:lifeskim |
| pubmed-article:16030218 | lifeskim:mentions | umls-concept:C0037473 | lld:lifeskim |
| pubmed-article:16030218 | lifeskim:mentions | umls-concept:C0052088 | lld:lifeskim |
| pubmed-article:16030218 | lifeskim:mentions | umls-concept:C0699748 | lld:lifeskim |
| pubmed-article:16030218 | lifeskim:mentions | umls-concept:C1314939 | lld:lifeskim |
| pubmed-article:16030218 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:16030218 | pubmed:issue | 15 | lld:pubmed |
| pubmed-article:16030218 | pubmed:dateCreated | 2005-7-20 | lld:pubmed |
| pubmed-article:16030218 | pubmed:abstractText | Sha (also known as Mrp/Mnh/Pha) is a Na+/H+ antiporter encoded by a cluster of six or seven genes that probably form a multisubunit transport complex. The Sha system is important for the homeostasis of H+, Na+, and other monovalent cations and plays a critical role in various functions, including alkaliphily, sporulation, and symbiosis. Here, we characterized the sha homologue genes from the opportunistic pathogen Pseudomonas aeruginosa, which exist as a cluster of six genes (PA1054 to PA1059). The gene cluster PA1054 to PA1059, but not the cluster with a deletion of PA1054, complemented a growth defect in the presence of 0.2 M NaCl and a defect in Na+/H+ antiport activity of the Escherichia coli TO114 mutant lacking the three major Na+/H+ antiporters, indicating that genes PA1054 to PA1059 are responsible for Na+/H+ antiport activity. We disrupted PA1054 (a shaA homologue gene) and determined its effect on Na+ tolerance during growth, Na+ efflux, and pathogenicity in mice. Disruption of PA1054 resulted in severe Na+ sensitivity during growth and decreased Na+ efflux activity. In mice, the deletion mutant of PA1054 also exhibited an attenuated virulence in systemic, pulmonary, and urinary tract infections and also a decrease in colonization of the infected organs. From these results, we conclude that the genes PA1054 to PA1059 encode a Na+/H+ antiporter that is largely responsible for Na+ extrusion in P. aeruginosa and has a role in the infection of the pathogen. We propose to designate PA1054 to PA1059 as the sha (sodium hydrogen antiporter) genes, shaABCDEFG. | lld:pubmed |
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| pubmed-article:16030218 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16030218 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16030218 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16030218 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16030218 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:16030218 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16030218 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16030218 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:16030218 | pubmed:issn | 0021-9193 | lld:pubmed |
| pubmed-article:16030218 | pubmed:author | pubmed-author:KudoToshiakiT | lld:pubmed |
| pubmed-article:16030218 | pubmed:author | pubmed-author:HagaKokiK | lld:pubmed |
| pubmed-article:16030218 | pubmed:author | pubmed-author:HatanoKazuoK | lld:pubmed |
| pubmed-article:16030218 | pubmed:author | pubmed-author:TakedaShinobu... | lld:pubmed |
| pubmed-article:16030218 | pubmed:author | pubmed-author:HinoMotohiroM | lld:pubmed |
| pubmed-article:16030218 | pubmed:author | pubmed-author:KosonoSaoriS | lld:pubmed |
| pubmed-article:16030218 | pubmed:author | pubmed-author:TomizawaRuiR | lld:pubmed |
| pubmed-article:16030218 | pubmed:author | pubmed-author:KajiyamaYusuk... | lld:pubmed |
| pubmed-article:16030218 | pubmed:author | pubmed-author:WakaiYoshimiY | lld:pubmed |
| pubmed-article:16030218 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16030218 | pubmed:volume | 187 | lld:pubmed |
| pubmed-article:16030218 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16030218 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16030218 | pubmed:pagination | 5242-8 | lld:pubmed |
| pubmed-article:16030218 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:16030218 | pubmed:meshHeading | pubmed-meshheading:16030218... | lld:pubmed |
| pubmed-article:16030218 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:16030218 | pubmed:articleTitle | Characterization of a multigene-encoded sodium/hydrogen antiporter (sha) from Pseudomonas aeruginosa: its involvement in pathogenesis. | lld:pubmed |
| pubmed-article:16030218 | pubmed:affiliation | Environmental Molecular Biology Laboratory, RIKEN, Wako, Saitama 351-0198, Japan. kosono@riken.jp | lld:pubmed |
| pubmed-article:16030218 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16030218 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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