pubmed-article:16024622 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:16024622 | lifeskim:mentions | umls-concept:C0032659 | lld:lifeskim |
pubmed-article:16024622 | lifeskim:mentions | umls-concept:C1332002 | lld:lifeskim |
pubmed-article:16024622 | lifeskim:mentions | umls-concept:C0334227 | lld:lifeskim |
pubmed-article:16024622 | pubmed:issue | 14 | lld:pubmed |
pubmed-article:16024622 | pubmed:dateCreated | 2005-7-18 | lld:pubmed |
pubmed-article:16024622 | pubmed:abstractText | Recently, several human cancers including leukemia and breast and brain tumors were found to contain stem-like cancer cells called cancer stem cells (CSC). Most of these CSCs were identified using markers that identify putative normal stem cells. In some cases, stem-like cancer cells were identified using the flow cytometry-based side population technique. In this study, we first show that approximately 30% of cultured human cancer cells and xenograft tumors examined ( approximately 30 in total) possess a detectable side population. Purified side population cells from two cell lines (U373 glioma and MCF7 breast cancer) and a xenograft prostate tumor (LAPC-9) are more tumorigenic than the corresponding non-side population cells. These side population cells also possess some intrinsic stem cell properties as they generate non-side population cells in vivo, can be further transplanted, and preferentially express some "stemness" genes, including Notch-1 and beta-catenin. Because the side population phenotype is mainly mediated by ABCG2, an ATP-binding cassette half-transporter associated with multidrug resistance, we subsequently studied ABCG2+ and ABCG2- cancer cells with respect to their tumorigenicity in vivo. Although side population cells show increased ABCG2 mRNA expression relative to the non-side population cells and all cancer cells and xenograft tumors examined express ABCG2 in a small fraction (0.5-3%) of the cells, highly purified ABCG2+ cancer cells, surprisingly, have very similar tumorigenicity to the ABCG2- cancer cells. Mechanistic studies indicate that ABCG2 expression is associated with proliferation and ABCG2+ cancer cells can generate ABCG2- cells. However, ABCG2- cancer cells can also generate ABCG2+ cells. Furthermore, the ABCG2- cancer cells form more and larger clones in the long-term clonal analyses and the ABCG2- population preferentially expresses several "stemness" genes. Taken together, our results suggest that (a) the side population is enriched with tumorigenic stem-like cancer cells, (b) ABCG2 expression identifies mainly fast-cycling tumor progenitors, and (c) the ABCG2- population contains primitive stem-like cancer cells. | lld:pubmed |
pubmed-article:16024622 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:16024622 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
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pubmed-article:16024622 | pubmed:language | eng | lld:pubmed |
pubmed-article:16024622 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:16024622 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:16024622 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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pubmed-article:16024622 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:16024622 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:16024622 | pubmed:month | Jul | lld:pubmed |
pubmed-article:16024622 | pubmed:issn | 0008-5472 | lld:pubmed |
pubmed-article:16024622 | pubmed:author | pubmed-author:TangDean GDG | lld:pubmed |
pubmed-article:16024622 | pubmed:author | pubmed-author:ClaypoolKentK | lld:pubmed |
pubmed-article:16024622 | pubmed:author | pubmed-author:ZhouJianjunJ | lld:pubmed |
pubmed-article:16024622 | pubmed:author | pubmed-author:PatrawalaLubn... | lld:pubmed |
pubmed-article:16024622 | pubmed:author | pubmed-author:CalhounTammyT | lld:pubmed |
pubmed-article:16024622 | pubmed:author | pubmed-author:Schneider-Bro... | lld:pubmed |
pubmed-article:16024622 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:16024622 | pubmed:day | 15 | lld:pubmed |
pubmed-article:16024622 | pubmed:volume | 65 | lld:pubmed |
pubmed-article:16024622 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:16024622 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:16024622 | pubmed:pagination | 6207-19 | lld:pubmed |
pubmed-article:16024622 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
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pubmed-article:16024622 | pubmed:meshHeading | pubmed-meshheading:16024622... | lld:pubmed |
pubmed-article:16024622 | pubmed:year | 2005 | lld:pubmed |
pubmed-article:16024622 | pubmed:articleTitle | Side population is enriched in tumorigenic, stem-like cancer cells, whereas ABCG2+ and ABCG2- cancer cells are similarly tumorigenic. | lld:pubmed |
pubmed-article:16024622 | pubmed:affiliation | Department of Carcinogenesis, Science Park-Research Division, The University of Texas M.D. Anderson Cancer Center, Smithville, Texas 78957, USA. | lld:pubmed |
pubmed-article:16024622 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:16024622 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
pubmed-article:16024622 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
pubmed-article:16024622 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
pubmed-article:16024622 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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