| pubmed-article:16023306 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16023306 | lifeskim:mentions | umls-concept:C0019704 | lld:lifeskim |
| pubmed-article:16023306 | lifeskim:mentions | umls-concept:C0024662 | lld:lifeskim |
| pubmed-article:16023306 | lifeskim:mentions | umls-concept:C0035668 | lld:lifeskim |
| pubmed-article:16023306 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:16023306 | pubmed:dateCreated | 2005-9-16 | lld:pubmed |
| pubmed-article:16023306 | pubmed:abstractText | We present evidence that the HIV-1 Rev protein can heterologously regulate expression of the simple beta retrovirus mouse mammary tumour virus (MMTV). Up to 10-fold upregulation was seen in a functional assay system when specific MMTV sequences were substituted for the HIV-1 Rev responsive element (RRE). RNA gel shift analysis showed that purified recombinant Rev could specifically bind to MMTV unique region 3 prime (U3) RNA and that these sequences could compete for wild-type Rev-RRE binding approximately 20-fold more efficiently than a non-specific competitor RNA. Using a combination of in silico and deletion mutation analyses, it was not possible to define any single specific secondary structure responsive to Rev, suggesting that a structure or combination of structures that only form in the context of the complete U3 transcript is/are required to interact with Rev. Taken together, these results suggest that HIV-1 Rev can directly bind to MMTV RNA as well as mediate upregulation of MMTV gene expression. | lld:pubmed |
| pubmed-article:16023306 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16023306 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16023306 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16023306 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16023306 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16023306 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16023306 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16023306 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:16023306 | pubmed:issn | 0378-1119 | lld:pubmed |
| pubmed-article:16023306 | pubmed:author | pubmed-author:SalmonsBrianB | lld:pubmed |
| pubmed-article:16023306 | pubmed:author | pubmed-author:GünzburgWalte... | lld:pubmed |
| pubmed-article:16023306 | pubmed:author | pubmed-author:EgerbacherMon... | lld:pubmed |
| pubmed-article:16023306 | pubmed:author | pubmed-author:HohenadlChris... | lld:pubmed |
| pubmed-article:16023306 | pubmed:author | pubmed-author:DangerfieldJo... | lld:pubmed |
| pubmed-article:16023306 | pubmed:author | pubmed-author:KodajovaPetra... | lld:pubmed |
| pubmed-article:16023306 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16023306 | pubmed:day | 26 | lld:pubmed |
| pubmed-article:16023306 | pubmed:volume | 358 | lld:pubmed |
| pubmed-article:16023306 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16023306 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16023306 | pubmed:pagination | 17-30 | lld:pubmed |
| pubmed-article:16023306 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:16023306 | pubmed:meshHeading | pubmed-meshheading:16023306... | lld:pubmed |
| pubmed-article:16023306 | pubmed:meshHeading | pubmed-meshheading:16023306... | lld:pubmed |
| pubmed-article:16023306 | pubmed:meshHeading | pubmed-meshheading:16023306... | lld:pubmed |
| pubmed-article:16023306 | pubmed:meshHeading | pubmed-meshheading:16023306... | lld:pubmed |
| pubmed-article:16023306 | pubmed:meshHeading | pubmed-meshheading:16023306... | lld:pubmed |
| pubmed-article:16023306 | pubmed:meshHeading | pubmed-meshheading:16023306... | lld:pubmed |
| pubmed-article:16023306 | pubmed:meshHeading | pubmed-meshheading:16023306... | lld:pubmed |
| pubmed-article:16023306 | pubmed:meshHeading | pubmed-meshheading:16023306... | lld:pubmed |
| pubmed-article:16023306 | pubmed:meshHeading | pubmed-meshheading:16023306... | lld:pubmed |
| pubmed-article:16023306 | pubmed:meshHeading | pubmed-meshheading:16023306... | lld:pubmed |
| pubmed-article:16023306 | pubmed:meshHeading | pubmed-meshheading:16023306... | lld:pubmed |
| pubmed-article:16023306 | pubmed:meshHeading | pubmed-meshheading:16023306... | lld:pubmed |
| pubmed-article:16023306 | pubmed:meshHeading | pubmed-meshheading:16023306... | lld:pubmed |
| pubmed-article:16023306 | pubmed:meshHeading | pubmed-meshheading:16023306... | lld:pubmed |
| pubmed-article:16023306 | pubmed:meshHeading | pubmed-meshheading:16023306... | lld:pubmed |
| pubmed-article:16023306 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:16023306 | pubmed:articleTitle | HIV-1 Rev can specifically interact with MMTV RNA and upregulate gene expression. | lld:pubmed |
| pubmed-article:16023306 | pubmed:affiliation | Research Institute of Virology and Biomedicine, University of Veterinary Medicine, Vienna, Austria. john.dangerfield@vu-wien.ac.at | lld:pubmed |
| pubmed-article:16023306 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16023306 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16023306 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16023306 | lld:pubmed |
