| pubmed-article:16014562 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16014562 | lifeskim:mentions | umls-concept:C0042971 | lld:lifeskim |
| pubmed-article:16014562 | lifeskim:mentions | umls-concept:C0031437 | lld:lifeskim |
| pubmed-article:16014562 | lifeskim:mentions | umls-concept:C1413036 | lld:lifeskim |
| pubmed-article:16014562 | lifeskim:mentions | umls-concept:C1282971 | lld:lifeskim |
| pubmed-article:16014562 | lifeskim:mentions | umls-concept:C0011155 | lld:lifeskim |
| pubmed-article:16014562 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:16014562 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:16014562 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:16014562 | pubmed:dateCreated | 2005-10-20 | lld:pubmed |
| pubmed-article:16014562 | pubmed:abstractText | Von Willebrand factor (VWF) is unable to interact spontaneously with platelets because this interaction requires a conversion of the VWF A1 domain into a glycoprotein Ibalpha (GpIbalpha) binding conformation. Here, we discuss a llama-derived antibody fragment (AU/VWFa-11) that specifically recognizes the GpIbalpha-binding conformation. AU/VWFa-11 is unable to bind VWF in solution, but efficiently interacts with ristocetin- or botrocetin-activated VWF, VWF comprising type 2B mutation R1306Q, or immobilized VWF. These unique properties allowed us to use AU/VWFa-11 for the detection of activated VWF in plasma of patients characterized by spontaneous VWF-platelet interactions: von Willebrand disease (VWD) type 2B and thrombotic thrombocytopenic purpura (TTP). For VWD type 2B, levels of activated VWF were increased 12-fold (P < .001) compared to levels in healthy volunteers. An inverse correlation between activated VWF levels and platelet count was observed (R2 = 0.74; P < .003). With regard to TTP, a 2-fold (P < .001) increase in activated VWF levels was found in plasma of patients with acquired TTP, whereas an 8-fold increase (P < .003) was found in congenital TTP. No overlap in levels of activated VWF could be detected between acquired and congenital TTP, suggesting that AU/VWFa-11 could be used to distinguish between both disorders. Furthermore, it could provide a tool to investigate the role of VWF in the development of thrombocytopenia in various diseases. | lld:pubmed |
| pubmed-article:16014562 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16014562 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16014562 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:16014562 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16014562 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16014562 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16014562 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16014562 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16014562 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16014562 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16014562 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:16014562 | pubmed:issn | 0006-4971 | lld:pubmed |
| pubmed-article:16014562 | pubmed:author | pubmed-author:LentingPeter... | lld:pubmed |
| pubmed-article:16014562 | pubmed:author | pubmed-author:de... | lld:pubmed |
| pubmed-article:16014562 | pubmed:author | pubmed-author:FijnheerRobR | lld:pubmed |
| pubmed-article:16014562 | pubmed:author | pubmed-author:VeyradierAgnè... | lld:pubmed |
| pubmed-article:16014562 | pubmed:author | pubmed-author:SilenceKarenK | lld:pubmed |
| pubmed-article:16014562 | pubmed:author | pubmed-author:HulsteinJanin... | lld:pubmed |
| pubmed-article:16014562 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16014562 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:16014562 | pubmed:volume | 106 | lld:pubmed |
| pubmed-article:16014562 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16014562 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16014562 | pubmed:pagination | 3035-42 | lld:pubmed |
| pubmed-article:16014562 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:16014562 | pubmed:meshHeading | pubmed-meshheading:16014562... | lld:pubmed |
| pubmed-article:16014562 | pubmed:meshHeading | pubmed-meshheading:16014562... | lld:pubmed |
| pubmed-article:16014562 | pubmed:meshHeading | pubmed-meshheading:16014562... | lld:pubmed |
| pubmed-article:16014562 | pubmed:meshHeading | pubmed-meshheading:16014562... | lld:pubmed |
| pubmed-article:16014562 | pubmed:meshHeading | pubmed-meshheading:16014562... | lld:pubmed |
| pubmed-article:16014562 | pubmed:meshHeading | pubmed-meshheading:16014562... | lld:pubmed |
| pubmed-article:16014562 | pubmed:meshHeading | pubmed-meshheading:16014562... | lld:pubmed |
| pubmed-article:16014562 | pubmed:meshHeading | pubmed-meshheading:16014562... | lld:pubmed |
| pubmed-article:16014562 | pubmed:meshHeading | pubmed-meshheading:16014562... | lld:pubmed |
| pubmed-article:16014562 | pubmed:meshHeading | pubmed-meshheading:16014562... | lld:pubmed |
| pubmed-article:16014562 | pubmed:meshHeading | pubmed-meshheading:16014562... | lld:pubmed |
| pubmed-article:16014562 | pubmed:meshHeading | pubmed-meshheading:16014562... | lld:pubmed |
| pubmed-article:16014562 | pubmed:meshHeading | pubmed-meshheading:16014562... | lld:pubmed |
| pubmed-article:16014562 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:16014562 | pubmed:articleTitle | A novel nanobody that detects the gain-of-function phenotype of von Willebrand factor in ADAMTS13 deficiency and von Willebrand disease type 2B. | lld:pubmed |
| pubmed-article:16014562 | pubmed:affiliation | Laboratory for Thrombosis and Haemostasis, Department of Haematology, G03.647, University Medical Center Utrecht, PO Box 85500, 3584 CX Utrecht, The Netherlands. | lld:pubmed |
| pubmed-article:16014562 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16014562 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16014562 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16014562 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16014562 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16014562 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16014562 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16014562 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16014562 | lld:pubmed |
