| pubmed-article:1600611 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1600611 | lifeskim:mentions | umls-concept:C0034493 | lld:lifeskim |
| pubmed-article:1600611 | lifeskim:mentions | umls-concept:C0003250 | lld:lifeskim |
| pubmed-article:1600611 | lifeskim:mentions | umls-concept:C0020960 | lld:lifeskim |
| pubmed-article:1600611 | lifeskim:mentions | umls-concept:C0596040 | lld:lifeskim |
| pubmed-article:1600611 | lifeskim:mentions | umls-concept:C0596751 | lld:lifeskim |
| pubmed-article:1600611 | lifeskim:mentions | umls-concept:C0185125 | lld:lifeskim |
| pubmed-article:1600611 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:1600611 | pubmed:dateCreated | 1992-7-14 | lld:pubmed |
| pubmed-article:1600611 | pubmed:abstractText | Monoclonal antibodies and rabbit antisera were produced that recognized 4-aminobiphenyl, its major DNA adducts and other metabolites. The antigens used to raise these antibodies were synthesized by coupling the aromatic amine to protein through a diazotization reaction. The goal of this immunization strategy was to induce antibodies that also cross-reacted with most 4-aminobiphenyl-derived metabolites. A total of 20 mice and four rabbits were immunized and every animal produced a strong immune response for 4-aminobiphenyl and its derivatives. Two IgG1 monoclonal antibodies, 3D6 and 2E11, were isolated from two different mouse spleen cell fusions. One of the monoclonal antibodies, 3D6, had a high recognition for the three major 4-aminobiphenyl-DNA adducts: N-(deoxyguanosine-8-yl)-4-aminobiphenyl, N-(deoxyadenosin-8-yl)-4-aminobiphenyl and N-(deoxyguanosine-N2-yl)-4-aminobiphenyl, with affinity constants between 2 and 4 x 10(9) l/mol. In addition, one of the rabbit anti-sera had an affinity constant for the DNA adducts of 2.1 x 10(9) l/mole. Thus, the strategy to use a diazotization coupling reaction was successful at producing high-affinity aminobiphenyl-DNA adduct-specific antibodies. Preparative immunoaffinity resins were made for each monoclonal antibody. These resins quantitatively bound 500 ng each [3H]N-acetyl-aminobiphenyl, [3H]N-aminobiphenyl and [3H]N-(deoxyguanosine-8-yl)-4-aminobiphenyl. Preliminary experiments were performed to test the applicability of the preparative monoclonal antibody immunoaffinity column to isolate [3H]4-aminobiphenyl-derived metabolites in dosed rat and dog urine. About 70% of the radioactivity in rat or dog urine could be bound to the immunoaffinity columns. The combined immunoaffinity column/HPLC analysis of the dog urine led to the identification of a novel urinary metabolite, N-formyl-aminobiphenyl. HPLC analysis of a rat urine sample tentatively found 4-aminobiphenyl, N-acetyl-4-aminobiphenyl and N-formyl-4-aminobiphenyl by co-chromatography, and these compounds accounted for 20, 6.8 and 6.5% of the total radioactivity in the chromatogram respectively. Taken together, these data show that these 4-aminobiphenyl-specific monoclonal antibodies can be used in immunoaffinity columns to isolate metabolites and DNA adducts from biological samples. | lld:pubmed |
| pubmed-article:1600611 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1600611 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1600611 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1600611 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1600611 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1600611 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1600611 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1600611 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1600611 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1600611 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1600611 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1600611 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1600611 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:1600611 | pubmed:issn | 0143-3334 | lld:pubmed |
| pubmed-article:1600611 | pubmed:author | pubmed-author:TannenbaumS... | lld:pubmed |
| pubmed-article:1600611 | pubmed:author | pubmed-author:KadlubarF FFF | lld:pubmed |
| pubmed-article:1600611 | pubmed:author | pubmed-author:SkipperP LPL | lld:pubmed |
| pubmed-article:1600611 | pubmed:author | pubmed-author:GroopmanJ DJD | lld:pubmed |
| pubmed-article:1600611 | pubmed:author | pubmed-author:DonahueP RPR | lld:pubmed |
| pubmed-article:1600611 | pubmed:author | pubmed-author:TrudelL JLJ | lld:pubmed |
| pubmed-article:1600611 | pubmed:author | pubmed-author:WildschutteMM | lld:pubmed |
| pubmed-article:1600611 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1600611 | pubmed:volume | 13 | lld:pubmed |
| pubmed-article:1600611 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1600611 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1600611 | pubmed:pagination | 917-22 | lld:pubmed |
| pubmed-article:1600611 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:1600611 | pubmed:meshHeading | pubmed-meshheading:1600611-... | lld:pubmed |
| pubmed-article:1600611 | pubmed:meshHeading | pubmed-meshheading:1600611-... | lld:pubmed |
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| pubmed-article:1600611 | pubmed:meshHeading | pubmed-meshheading:1600611-... | lld:pubmed |
| pubmed-article:1600611 | pubmed:meshHeading | pubmed-meshheading:1600611-... | lld:pubmed |
| pubmed-article:1600611 | pubmed:meshHeading | pubmed-meshheading:1600611-... | lld:pubmed |
| pubmed-article:1600611 | pubmed:meshHeading | pubmed-meshheading:1600611-... | lld:pubmed |
| pubmed-article:1600611 | pubmed:meshHeading | pubmed-meshheading:1600611-... | lld:pubmed |
| pubmed-article:1600611 | pubmed:meshHeading | pubmed-meshheading:1600611-... | lld:pubmed |
| pubmed-article:1600611 | pubmed:meshHeading | pubmed-meshheading:1600611-... | lld:pubmed |
| pubmed-article:1600611 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1600611 | pubmed:articleTitle | Monoclonal antibodies and rabbit antisera recognizing 4-aminobiphenyl--DNA adducts and application to immunoaffinity chromatography. | lld:pubmed |
| pubmed-article:1600611 | pubmed:affiliation | Department of Environmental Health Sciences, Johns Hopkins University, School of Hygiene and Public Health, Baltimore, MD 21205. | lld:pubmed |
| pubmed-article:1600611 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1600611 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1600611 | lld:pubmed |
