| pubmed-article:15998740 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15998740 | lifeskim:mentions | umls-concept:C1095831 | lld:lifeskim |
| pubmed-article:15998740 | lifeskim:mentions | umls-concept:C0004755 | lld:lifeskim |
| pubmed-article:15998740 | lifeskim:mentions | umls-concept:C0008633 | lld:lifeskim |
| pubmed-article:15998740 | lifeskim:mentions | umls-concept:C1705914 | lld:lifeskim |
| pubmed-article:15998740 | lifeskim:mentions | umls-concept:C0205360 | lld:lifeskim |
| pubmed-article:15998740 | pubmed:issue | 28 | lld:pubmed |
| pubmed-article:15998740 | pubmed:dateCreated | 2005-7-14 | lld:pubmed |
| pubmed-article:15998740 | pubmed:abstractText | The satellite sequences (AGGGAG)(n) and Ty3/gypsy-like retrotransposons are known to localize at the barley centromeres. Using a gametocidal system, which induces chromosomal mutations in barley chromosomes added to common wheat, we obtained an isochromosome for the short arm of barley chromosome 7H (7HS) that lacked the barley-specific satellite sequence (AGGGAG)(n). Two telocentric derivatives of the isochromosome arose in the progeny: 7HS* with and 7HS** without the pericentromeric C-band. FISH analysis demonstrated that both telosomes lacked not only the barley-specific centromeric (AGGGAG)(n) repeats and retroelements but also any of the known wheat centromeric tandem repeats, including the 192-bp, 250-bp, and TaiI sequences. Although they lacked these centromeric repeats, 7HS* and 7HS** both showed normal mitotic and meiotic transmission. Translocation of barley centromeric repeats to a wheat chromosome 4A did not generate a dicentric chromosome. Indirect immunostaining revealed that all tested centromere-specific proteins (rice CENH3, maize CENP-C, and putative barley homologues of the yeast kinetochore proteins CBF5 and SKP1) and histone H3 phosphorylated at serines 10 and 28 localized at the centromeric region of 7HS*. We conclude that the barley centromeric repeats are neither sufficient nor obligatory to assemble kinetochores, and we discuss the possible formation of a novel centromere in a barley chromosome. | lld:pubmed |
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| pubmed-article:15998740 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:15998740 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:15998740 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15998740 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:15998740 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:15998740 | pubmed:author | pubmed-author:HoubenAA | lld:pubmed |
| pubmed-article:15998740 | pubmed:author | pubmed-author:SchubertII | lld:pubmed |
| pubmed-article:15998740 | pubmed:author | pubmed-author:EndoT RTR | lld:pubmed |
| pubmed-article:15998740 | pubmed:author | pubmed-author:NasudaSS | lld:pubmed |
| pubmed-article:15998740 | pubmed:author | pubmed-author:HudakovaSS | lld:pubmed |
| pubmed-article:15998740 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15998740 | pubmed:day | 12 | lld:pubmed |
| pubmed-article:15998740 | pubmed:volume | 102 | lld:pubmed |
| pubmed-article:15998740 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15998740 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15998740 | pubmed:pagination | 9842-7 | lld:pubmed |
| pubmed-article:15998740 | pubmed:dateRevised | 2010-9-20 | lld:pubmed |
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| pubmed-article:15998740 | pubmed:meshHeading | pubmed-meshheading:15998740... | lld:pubmed |
| pubmed-article:15998740 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15998740 | pubmed:articleTitle | Stable barley chromosomes without centromeric repeats. | lld:pubmed |
| pubmed-article:15998740 | pubmed:affiliation | Laboratory of Plant Genetics, Graduate School of Agriculture and Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan. | lld:pubmed |
| pubmed-article:15998740 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15998740 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:15998740 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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