| pubmed-article:15970625 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15970625 | lifeskim:mentions | umls-concept:C0026609 | lld:lifeskim |
| pubmed-article:15970625 | lifeskim:mentions | umls-concept:C0037657 | lld:lifeskim |
| pubmed-article:15970625 | lifeskim:mentions | umls-concept:C0011209 | lld:lifeskim |
| pubmed-article:15970625 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:15970625 | pubmed:issue | 2-3 | lld:pubmed |
| pubmed-article:15970625 | pubmed:dateCreated | 2005-6-22 | lld:pubmed |
| pubmed-article:15970625 | pubmed:abstractText | Recent work has demonstrated that adeno-associated viral (AAV) vector-mediated delivery of the insulin-like growth factor (IGF-I) gene through retrograde axonal transport can prolong survival and delay disease onset in the superoxide dismutase mutant mouse model of motor neuron (MN) disease. The present experiment examines IGF-I gene transfer in vitro. Adenoviral and AAV vectors for IGF-I infect neurons triggering expression and secretion of biologically active IGF-I. AAV-mediated IGF-I expression in SH-SY5Y neurons protects both cells expressing the transgene, and bystanders without transgene expression from glutamate-induced apoptosis. Similarly, AAV-mediated IGF-I delivery in primary E15 MN culture provides a titer-dependent neuroprotection from glutamate-induced DNA fragmentation. Both infected and noninfected neurons are equally protected. These observations argue that vector-mediated IGF-I gene transfer induces secretion of active IGF-I that acts through direct effects on spinal cord MNs. This mechanism may explain the therapeutic effects observed in vivo despite relatively low affinity AAV spinal cord uptake. | lld:pubmed |
| pubmed-article:15970625 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15970625 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15970625 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15970625 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15970625 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15970625 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15970625 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15970625 | pubmed:issn | 1535-1084 | lld:pubmed |
| pubmed-article:15970625 | pubmed:author | pubmed-author:ZhangWeiW | lld:pubmed |
| pubmed-article:15970625 | pubmed:author | pubmed-author:SongDebbie... | lld:pubmed |
| pubmed-article:15970625 | pubmed:author | pubmed-author:BoulisNichola... | lld:pubmed |
| pubmed-article:15970625 | pubmed:author | pubmed-author:FeldmanEva... | lld:pubmed |
| pubmed-article:15970625 | pubmed:author | pubmed-author:VincentAndrea... | lld:pubmed |
| pubmed-article:15970625 | pubmed:author | pubmed-author:ImperialeMich... | lld:pubmed |
| pubmed-article:15970625 | pubmed:author | pubmed-author:SchildAndreas... | lld:pubmed |
| pubmed-article:15970625 | pubmed:author | pubmed-author:JungVerenaV | lld:pubmed |
| pubmed-article:15970625 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15970625 | pubmed:volume | 6 | lld:pubmed |
| pubmed-article:15970625 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15970625 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15970625 | pubmed:pagination | 79-85 | lld:pubmed |
| pubmed-article:15970625 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:15970625 | pubmed:meshHeading | pubmed-meshheading:15970625... | lld:pubmed |
| pubmed-article:15970625 | pubmed:meshHeading | pubmed-meshheading:15970625... | lld:pubmed |
| pubmed-article:15970625 | pubmed:meshHeading | pubmed-meshheading:15970625... | lld:pubmed |
| pubmed-article:15970625 | pubmed:meshHeading | pubmed-meshheading:15970625... | lld:pubmed |
| pubmed-article:15970625 | pubmed:meshHeading | pubmed-meshheading:15970625... | lld:pubmed |
| pubmed-article:15970625 | pubmed:meshHeading | pubmed-meshheading:15970625... | lld:pubmed |
| pubmed-article:15970625 | pubmed:meshHeading | pubmed-meshheading:15970625... | lld:pubmed |
| pubmed-article:15970625 | pubmed:meshHeading | pubmed-meshheading:15970625... | lld:pubmed |
| pubmed-article:15970625 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15970625 | pubmed:articleTitle | Adeno-associated viral-mediated insulin-like growth factor delivery protects motor neurons in vitro. | lld:pubmed |
| pubmed-article:15970625 | pubmed:affiliation | Department of Neurology, University of Michigan, MI, USA. | lld:pubmed |
| pubmed-article:15970625 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15970625 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:15970625 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:15970625 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
| entrez-gene:16000 | entrezgene:pubmed | pubmed-article:15970625 | lld:entrezgene |
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