| pubmed-article:1596288 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1596288 | lifeskim:mentions | umls-concept:C0033634 | lld:lifeskim |
| pubmed-article:1596288 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:1596288 | lifeskim:mentions | umls-concept:C0165168 | lld:lifeskim |
| pubmed-article:1596288 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:1596288 | pubmed:dateCreated | 1992-6-26 | lld:pubmed |
| pubmed-article:1596288 | pubmed:abstractText | The bryostatins represent a unique class of activators of protein kinase C (PKC) which induce only a subset of the responses typical of the phorbol esters and block those responses to the phorbol esters which they themselves do not induce. To better understand the interaction of the bryostatins with PKC, we have synthesized [26-3H]bryostatin 4 and characterized its binding to PKC. [3H]Bryostatin 4 and [3H]phorbol 12,13-dibutyrate ([3H]PDBu) differed markedly in their binding to PKC reconstituted with phosphatidylserine (PS). The binding affinity of [3H]bryostatin 4 under these conditions was too high to measure and the rate of release of bound bryostatin was much slower than that of the phorbol esters, with a half-time of several hours. These properties caused bryostatin 1 to appear to inhibit [3H]PDBu binding under these conditions in a non-competitive fashion. Both the high potency and the slow rate of release of the bryostatins may contribute to their unique pattern of biological activity. By reconstituting PKC in a mixture of 1.5% Triton X-100:0.3% PS, we were able to establish reversible conditions for [3H]bryostatin 4 binding. Under these latter conditions, binding of [3H]bryostatin 4 was competitively inhibited by PDBu, consistent with both the bryostatin and phorbol esters binding to PKC in a qualitatively similar fashion. Binding affinities to PKC isozymes alpha, beta, and gamma were compared and little difference was found, suggesting that differential recognition by these isozymes does not account for the unique biological activity of the bryostatins. | lld:pubmed |
| pubmed-article:1596288 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1596288 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1596288 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1596288 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1596288 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1596288 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1596288 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1596288 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1596288 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1596288 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1596288 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1596288 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1596288 | pubmed:month | May | lld:pubmed |
| pubmed-article:1596288 | pubmed:issn | 0006-2952 | lld:pubmed |
| pubmed-article:1596288 | pubmed:author | pubmed-author:LewisN JNJ | lld:pubmed |
| pubmed-article:1596288 | pubmed:author | pubmed-author:BlumbergP MPM | lld:pubmed |
| pubmed-article:1596288 | pubmed:author | pubmed-author:PettitG RGR | lld:pubmed |
| pubmed-article:1596288 | pubmed:author | pubmed-author:WarrenB SBS | lld:pubmed |
| pubmed-article:1596288 | pubmed:author | pubmed-author:Dell'AquilaM... | lld:pubmed |
| pubmed-article:1596288 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1596288 | pubmed:day | 8 | lld:pubmed |
| pubmed-article:1596288 | pubmed:volume | 43 | lld:pubmed |
| pubmed-article:1596288 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1596288 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1596288 | pubmed:pagination | 2007-14 | lld:pubmed |
| pubmed-article:1596288 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:1596288 | pubmed:meshHeading | pubmed-meshheading:1596288-... | lld:pubmed |
| pubmed-article:1596288 | pubmed:meshHeading | pubmed-meshheading:1596288-... | lld:pubmed |
| pubmed-article:1596288 | pubmed:meshHeading | pubmed-meshheading:1596288-... | lld:pubmed |
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| pubmed-article:1596288 | pubmed:meshHeading | pubmed-meshheading:1596288-... | lld:pubmed |
| pubmed-article:1596288 | pubmed:meshHeading | pubmed-meshheading:1596288-... | lld:pubmed |
| pubmed-article:1596288 | pubmed:meshHeading | pubmed-meshheading:1596288-... | lld:pubmed |
| pubmed-article:1596288 | pubmed:meshHeading | pubmed-meshheading:1596288-... | lld:pubmed |
| pubmed-article:1596288 | pubmed:meshHeading | pubmed-meshheading:1596288-... | lld:pubmed |
| pubmed-article:1596288 | pubmed:meshHeading | pubmed-meshheading:1596288-... | lld:pubmed |
| pubmed-article:1596288 | pubmed:meshHeading | pubmed-meshheading:1596288-... | lld:pubmed |
| pubmed-article:1596288 | pubmed:meshHeading | pubmed-meshheading:1596288-... | lld:pubmed |
| pubmed-article:1596288 | pubmed:meshHeading | pubmed-meshheading:1596288-... | lld:pubmed |
| pubmed-article:1596288 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1596288 | pubmed:articleTitle | Binding of [3H]bryostatin 4 to protein kinase C. | lld:pubmed |
| pubmed-article:1596288 | pubmed:affiliation | Molecular Mechanisms of Tumor Promotion Section, National Cancer Institute, Bethesda, MD 20892. | lld:pubmed |
| pubmed-article:1596288 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1596288 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:1596288 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1596288 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1596288 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1596288 | lld:pubmed |
