| pubmed-article:159451 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:159451 | lifeskim:mentions | umls-concept:C0028630 | lld:lifeskim |
| pubmed-article:159451 | lifeskim:mentions | umls-concept:C0038734 | lld:lifeskim |
| pubmed-article:159451 | lifeskim:mentions | umls-concept:C0205681 | lld:lifeskim |
| pubmed-article:159451 | lifeskim:mentions | umls-concept:C0027112 | lld:lifeskim |
| pubmed-article:159451 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:159451 | pubmed:dateCreated | 1980-1-28 | lld:pubmed |
| pubmed-article:159451 | pubmed:abstractText | Studies with reagents that crosslink two thiol groups have shown that it is possible to trap nucleotides at the active site of myosin chymotryptic subfragment 1. Subfragment 1 incorporates nearly stoichiometric quantities of [14C]ATP or [14C]ADP in a manner that depends linearly on the extent of inactivation by either N,N'-p-phenylenedimaleimide or Co(II)phenanthroline/[Co(III)(phenanthroline)2CO3]+ complexes. The incorporated radioactive nucleotide is retained after gel filtration, even when the enzyme derivatives are stored in the presence of EDTA or nonradioactive nucleotides (t 1/2 approximately 5 days). The nucleotide incorporated is not covalently bound because HClO4 denaturation allows immediate release of bound nucleotide. The nucleotide retained is ADP because the gamma-phosphate of [gamma-32P]ATP is lost after trapping. Subfragment 1 inactivated as above does not bind the competitive inhibitor adenosine 5'-[beta, gamma-imido]triphosphate, indicating that the active site is blocked. It is proposed that a jawlike nucleotide cleft closes on MgADP or MgATP, which can be locked shut by crosslinking two thiol groups by reaction with N,N'-p-phenylenedimaleimide or cobalt phenanthroline complexes. | lld:pubmed |
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| pubmed-article:159451 | pubmed:language | eng | lld:pubmed |
| pubmed-article:159451 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:159451 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:159451 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:159451 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:159451 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:159451 | pubmed:author | pubmed-author:YountR GRG | lld:pubmed |
| pubmed-article:159451 | pubmed:author | pubmed-author:WellsJ AJA | lld:pubmed |
| pubmed-article:159451 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:159451 | pubmed:volume | 76 | lld:pubmed |
| pubmed-article:159451 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:159451 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:159451 | pubmed:pagination | 4966-70 | lld:pubmed |
| pubmed-article:159451 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:159451 | pubmed:year | 1979 | lld:pubmed |
| pubmed-article:159451 | pubmed:articleTitle | Active site trapping of nucleotides by crosslinking two sulfhydryls in myosin subfragment 1. | lld:pubmed |
| pubmed-article:159451 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:159451 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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