| pubmed-article:1591245 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1591245 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:1591245 | lifeskim:mentions | umls-concept:C0597717 | lld:lifeskim |
| pubmed-article:1591245 | lifeskim:mentions | umls-concept:C0678594 | lld:lifeskim |
| pubmed-article:1591245 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:1591245 | lifeskim:mentions | umls-concept:C0205144 | lld:lifeskim |
| pubmed-article:1591245 | pubmed:issue | 20 | lld:pubmed |
| pubmed-article:1591245 | pubmed:dateCreated | 1992-6-30 | lld:pubmed |
| pubmed-article:1591245 | pubmed:abstractText | We have carried out fluorescence resonance energy transfer (FRET) measurements on four-way DNA junctions in order to analyze the global structure and its dependence on the concentration of several types of ions. A knowledge of the structure and its sensitivity to the solution environment is important for a full understanding of recombination events in DNA. The stereochemical arrangement of the four DNA helices that make up the four-way junction was established by a global comparison of the efficiency of FRET between donor and acceptor molecules attached pairwise in all possible permutations to the 5' termini of the duplex arms of the four-way structure. The conclusions are based upon a comparison between a series of many identical DNA molecules which have been labeled on different positions, rather than a determination of a few absolute distances. Details of the FRET analysis are presented; features of the analysis with particular relevance to DNA structures are emphasized. Three methods were employed to determine the efficiency of FRET: (1) enhancement of the acceptor fluorescence, (2) decrease of the donor quantum yield, and (3) shortening of the donor fluorescence lifetime. The FRET results indicate that the arms of the four-way junction are arranged in an antiparallel stacked X-structure when salt is added to the solution. The ion-related conformational change upon addition of salt to a solution originally at low ionic strength progresses in a continuous noncooperative manner as the ionic strength of the solution increases. The mode of ion interaction at the strand exchange site of the junction is discussed. | lld:pubmed |
| pubmed-article:1591245 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1591245 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:1591245 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:1591245 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1591245 | pubmed:month | May | lld:pubmed |
| pubmed-article:1591245 | pubmed:issn | 0006-2960 | lld:pubmed |
| pubmed-article:1591245 | pubmed:author | pubmed-author:LilleyD MDM | lld:pubmed |
| pubmed-article:1591245 | pubmed:author | pubmed-author:CleggR MRM | lld:pubmed |
| pubmed-article:1591245 | pubmed:author | pubmed-author:MurchieA IAI | lld:pubmed |
| pubmed-article:1591245 | pubmed:author | pubmed-author:DiekmannSS | lld:pubmed |
| pubmed-article:1591245 | pubmed:author | pubmed-author:ZechelAA | lld:pubmed |
| pubmed-article:1591245 | pubmed:author | pubmed-author:CarlbergCC | lld:pubmed |
| pubmed-article:1591245 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1591245 | pubmed:day | 26 | lld:pubmed |
| pubmed-article:1591245 | pubmed:volume | 31 | lld:pubmed |
| pubmed-article:1591245 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1591245 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1591245 | pubmed:pagination | 4846-56 | lld:pubmed |
| pubmed-article:1591245 | pubmed:dateRevised | 2009-9-29 | lld:pubmed |
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| pubmed-article:1591245 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1591245 | pubmed:articleTitle | Fluorescence resonance energy transfer analysis of the structure of the four-way DNA junction. | lld:pubmed |
| pubmed-article:1591245 | pubmed:affiliation | Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, Göttingen, Federal Republic of Germany. | lld:pubmed |
| pubmed-article:1591245 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1591245 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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