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pubmed-article:1590412pubmed:abstractTextIn vivo studies of fetal sheep suggest that the liquid present in the lumen of the lung throughout fetal life is derived from Cl- secretion by the pulmonary epithelium. Monolayer preparations of enriched epithelial cells from distal fetal rat (18-day gestation) lung, grown in serum-free media, were histologically similar to acinar (prealveolar) structures of fresh tissue. In Ussing chambers, basal transepithelial potential difference (PD), calculated equivalent short-circuit current (Ieq), and transepithelial resistance (R) were 4.4 +/- 0.3 mV (matrix positive), 35.6 +/- 1.6 microA/cm2, and 120.0 +/- 4.0 omega cm2, respectively. Ouabain (10(-3) M) eliminated 57% of basal Ieq within 30 min, amiloride (10(-4) M) induced a 13% fall in Ieq, and phlorizin (10(-4) M) had no effect on bioelectric properties. Diphenylamine-2-carboxylate (DPC, 3 x 10(-3) M) inhibited Ieq by 50%. Bumetanide had no effect on baseline bioelectric parameters. The hyperpolarization that accompanied apical or bilateral replacement of Cl- and was enhanced by terbutaline suggested an apical Cl- permselectivity. Effects of Na+ replacement on amiloride-pretreated monolayers were consistent with Na(+)-dependent Cl- secretion or amiloride-insensitive pathways. Under these growth conditions, this preparation exhibits bioelectric characteristics that are compatible with Cl- secretion and Na+ absorption. The mechanism of Cl- secretion may be similar to that of airways but is uniquely bumetanide insensitive.lld:pubmed
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pubmed-article:1590412pubmed:authorpubmed-author:GatzyJ TJTlld:pubmed
pubmed-article:1590412pubmed:authorpubmed-author:BoucherR CRClld:pubmed
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pubmed-article:1590412pubmed:authorpubmed-author:BarkerP MPMlld:pubmed
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pubmed-article:1590412pubmed:volume262lld:pubmed
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pubmed-article:1590412pubmed:paginationL628-36lld:pubmed
pubmed-article:1590412pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:1590412pubmed:articleTitleBioelectric properties of cultured epithelial monolayers from distal lung of 18-day fetal rat.lld:pubmed
pubmed-article:1590412pubmed:affiliationDepartment of Medicine, University of North Carolina, Chapel Hill 27599-7020.lld:pubmed
pubmed-article:1590412pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:1590412pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
pubmed-article:1590412pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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