| pubmed-article:15885434 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15885434 | lifeskim:mentions | umls-concept:C1514821 | lld:lifeskim |
| pubmed-article:15885434 | lifeskim:mentions | umls-concept:C0026019 | lld:lifeskim |
| pubmed-article:15885434 | lifeskim:mentions | umls-concept:C0450363 | lld:lifeskim |
| pubmed-article:15885434 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:15885434 | pubmed:dateCreated | 2005-5-11 | lld:pubmed |
| pubmed-article:15885434 | pubmed:abstractText | This paper presents an algorithm based on a continuous framework for a posteriori angular and translational assignment in three-dimensional electron microscopy (3DEM) of single particles. Our algorithm can be used advantageously to refine the assignment of standard quantized-parameter methods by registering the images to a reference 3D particle model. We achieve the registration by employing a gradient-based iterative minimization of a least-squares measure of dissimilarity between an image and a projection of the volume in the Fourier transform (FT) domain. We compute the FT of the projection using the central-slice theorem (CST). To compute the gradient accurately, we take advantage of a cubic B-spline model of the data in the frequency domain. To improve the robustness of the algorithm, we weight the cost function in the FT domain and apply a "mixed" strategy for the assignment based on the minimum value of the cost function at registration for several different initializations. We validate our algorithm in a fully controlled simulation environment. We show that the mixed strategy improves the assignment accuracy; on our data, the quality of the angular and translational assignment was better than 2 voxel (i.e., 6.54 angstroms). We also test the performance of our algorithm on real EM data. We conclude that our algorithm outperforms a standard projection-matching refinement in terms of both consistency of 3D reconstructions and speed. | lld:pubmed |
| pubmed-article:15885434 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15885434 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15885434 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15885434 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15885434 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15885434 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:15885434 | pubmed:issn | 0304-3991 | lld:pubmed |
| pubmed-article:15885434 | pubmed:author | pubmed-author:UnserMM | lld:pubmed |
| pubmed-article:15885434 | pubmed:author | pubmed-author:ThévenazPP | lld:pubmed |
| pubmed-article:15885434 | pubmed:author | pubmed-author:Joni?SS | lld:pubmed |
| pubmed-article:15885434 | pubmed:author | pubmed-author:De CarloSS | lld:pubmed |
| pubmed-article:15885434 | pubmed:author | pubmed-author:El-BezCC | lld:pubmed |
| pubmed-article:15885434 | pubmed:author | pubmed-author:SorzanoC O... | lld:pubmed |
| pubmed-article:15885434 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15885434 | pubmed:volume | 103 | lld:pubmed |
| pubmed-article:15885434 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15885434 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15885434 | pubmed:pagination | 303-17 | lld:pubmed |
| pubmed-article:15885434 | pubmed:meshHeading | pubmed-meshheading:15885434... | lld:pubmed |
| pubmed-article:15885434 | pubmed:meshHeading | pubmed-meshheading:15885434... | lld:pubmed |
| pubmed-article:15885434 | pubmed:meshHeading | pubmed-meshheading:15885434... | lld:pubmed |
| pubmed-article:15885434 | pubmed:meshHeading | pubmed-meshheading:15885434... | lld:pubmed |
| pubmed-article:15885434 | pubmed:meshHeading | pubmed-meshheading:15885434... | lld:pubmed |
| pubmed-article:15885434 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15885434 | pubmed:articleTitle | Spline-based image-to-volume registration for three-dimensional electron microscopy. | lld:pubmed |
| pubmed-article:15885434 | pubmed:affiliation | Biomedical Imaging Group, Ecole polytechnique fédérale de Lausanne (EPFL), CH-1015 Lausanne VD, Switzerland. Slavika.Jonic@lmcp.jussieu.fr | lld:pubmed |
| pubmed-article:15885434 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15885434 | pubmed:publicationType | Validation Studies | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15885434 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15885434 | lld:pubmed |
