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pubmed-article:15870374pubmed:abstractTextNumerous industrial bacteria generate hydrogen peroxide (H(2)O(2)), which may inhibit the growth of other bacteria in mixed ecosystems. We isolated spontaneous oxidative-stress-resistant (SpOx) Lactococcus lactis mutants by using a natural selection method with milk-adapted strains on dairy culture medium containing H(2)O(2). Three SpOx mutants displayed greater H(2)O(2) resistance. One of them, SpOx3, demonstrated better behavior in different oxidative-stress situations: (i) higher long-term survival upon aeration in LM17 and milk and (ii) the ability to grow with H(2)O(2)-producing Lactobacillus delbrueckii subsp. delbrueckii strains. Furthermore, the transit kinetics of the SpOx3 mutant in the digestive tract of a human flora-associated mouse model was not affected.lld:pubmed
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pubmed-article:15870374pubmed:dateRevised2009-11-18lld:pubmed
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pubmed-article:15870374pubmed:articleTitleLactococcus lactis SpOx spontaneous mutants: a family of oxidative-stress-resistant dairy strains.lld:pubmed
pubmed-article:15870374pubmed:affiliationUnité de Recherches Laitières et de Génetique, INRA, Domaine de Vilvert, 78352 Jouy en Josas cedex, France.lld:pubmed
pubmed-article:15870374pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:15870374pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed