| pubmed-article:15860771 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15860771 | lifeskim:mentions | umls-concept:C0078058 | lld:lifeskim |
| pubmed-article:15860771 | lifeskim:mentions | umls-concept:C0441889 | lld:lifeskim |
| pubmed-article:15860771 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:15860771 | lifeskim:mentions | umls-concept:C0040649 | lld:lifeskim |
| pubmed-article:15860771 | lifeskim:mentions | umls-concept:C1171892 | lld:lifeskim |
| pubmed-article:15860771 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:15860771 | lifeskim:mentions | umls-concept:C0301625 | lld:lifeskim |
| pubmed-article:15860771 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:15860771 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:15860771 | pubmed:dateCreated | 2005-4-29 | lld:pubmed |
| pubmed-article:15860771 | pubmed:abstractText | Gene expression is regulated at the transcriptional and post-transcriptional levels. Therefore, in order to achieve a high level of silencing, which includes minimizing any residual expression of a target gene, suppression at both the transcriptional and post-transcriptional levels is required. In this study, we describe a new method for highly efficient gene silencing that combines zinc finger protein-mediated transcriptional repression and small interfering RNA (siRNA)-mediated inhibition of post-transcriptional events. To measure the amount of gene expression under various conditions, we used a luciferase reporter gene that was driven by a variety of promoters, including that of the human vascular endothelial growth factor-A (VEGF-A) gene. We also measured expression of the endogenous VEGF-A gene. Inhibition of gene expression by each of the two individual technologies was effective, but in-depth analyses revealed residual expression of the target gene. The combination of specific zinc finger transcription factors and siRNAs greatly enhanced the silencing of the human VEGF-A gene, not only when cells were grown in the presence of normal amounts of oxygen but also under conditions of hypoxic stimulation. These results suggest that a bi-level approach to the silencing of VEGF-A expression may be clinically beneficial as part of a cancer treatment protocol. | lld:pubmed |
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| pubmed-article:15860771 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15860771 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15860771 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:15860771 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15860771 | pubmed:issn | 1362-4962 | lld:pubmed |
| pubmed-article:15860771 | pubmed:author | pubmed-author:CaliòF GFG | lld:pubmed |
| pubmed-article:15860771 | pubmed:author | pubmed-author:ShinHyun-Chul... | lld:pubmed |
| pubmed-article:15860771 | pubmed:author | pubmed-author:KwonHeung-Sun... | lld:pubmed |
| pubmed-article:15860771 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:15860771 | pubmed:volume | 33 | lld:pubmed |
| pubmed-article:15860771 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15860771 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15860771 | pubmed:pagination | e74 | lld:pubmed |
| pubmed-article:15860771 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:15860771 | pubmed:meshHeading | pubmed-meshheading:15860771... | lld:pubmed |
| pubmed-article:15860771 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15860771 | pubmed:articleTitle | Suppression of vascular endothelial growth factor expression at the transcriptional and post-transcriptional levels. | lld:pubmed |
| pubmed-article:15860771 | pubmed:affiliation | ToolGen, Inc. 461-6, Jeonmin-dong, Yuseong-gu, Daejeon 305-390, South Korea. | lld:pubmed |
| pubmed-article:15860771 | pubmed:publicationType | Journal Article | lld:pubmed |
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