| pubmed-article:15857612 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15857612 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:15857612 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:15857612 | lifeskim:mentions | umls-concept:C0026845 | lld:lifeskim |
| pubmed-article:15857612 | lifeskim:mentions | umls-concept:C0030851 | lld:lifeskim |
| pubmed-article:15857612 | lifeskim:mentions | umls-concept:C1325847 | lld:lifeskim |
| pubmed-article:15857612 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:15857612 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:15857612 | lifeskim:mentions | umls-concept:C0183014 | lld:lifeskim |
| pubmed-article:15857612 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:15857612 | pubmed:dateCreated | 2005-4-28 | lld:pubmed |
| pubmed-article:15857612 | pubmed:abstractText | Stimulation of the RhoA/Rho-kinase (ROK) signaling represents a key step in the maintenance of agonist-induced contraction of smooth muscle. We aimed to demonstrate Ca(2+) sensitization in rat anococcygeus and retractor penis muscles and to identify the molecular expression of major components of this pathway. Both anococcygeus and retractor penis showed a similar expression of RhoA, ROKalpha, and ROKbeta at the protein level as well as the mRNA for RhoGEFs. Cumulative addition of the ROK inhibitors H-1152 (0.001-3 microM), Y-27632 (0.01-30 microM) or HA-1077 (0.01-30 microM) caused sustained relaxations of precontracted smooth muscle strips. Ca(2+) sensitization induced by phenylephrine, norepinephrine and carbachol was markedly antagonized by all three ROK inhibitors. In addition, the contractile response to KCl-induced depolarization was highly sensitive to these ROK inhibitors. H-1152 was approximately 8-20 more potent than Y-27632 and HA-1077 to inhibit contraction. Electrical field stimulation (EFS, 1-32 Hz) caused transient contractions in both anococcygeus and retractor penis muscle, which were blocked by tetrodotoxin (1 microM), phentolamine (1 microM) or bretylium tosylate (30 microM). Similarly, H-1152 (0.1-1 microM), Y-27632 (1-10 microM) or HA-1077 (1-10 microM) significantly reduced EFS-evoked contractions in a concentration-dependent manner. The results indicate that the RhoA/ROK-mediated Ca(2+) sensitization pathway is expressed in anococcygeus and retractor penis muscles and enhances contractions produced by receptor-dependent and independent mechanisms. | lld:pubmed |
| pubmed-article:15857612 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15857612 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15857612 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15857612 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15857612 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15857612 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15857612 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:15857612 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15857612 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:15857612 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15857612 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15857612 | pubmed:month | May | lld:pubmed |
| pubmed-article:15857612 | pubmed:issn | 0006-2952 | lld:pubmed |
| pubmed-article:15857612 | pubmed:author | pubmed-author:WebbR... | lld:pubmed |
| pubmed-article:15857612 | pubmed:author | pubmed-author:TeixeiraClebe... | lld:pubmed |
| pubmed-article:15857612 | pubmed:author | pubmed-author:JinLimingL | lld:pubmed |
| pubmed-article:15857612 | pubmed:author | pubmed-author:YingZhekangZ | lld:pubmed |
| pubmed-article:15857612 | pubmed:author | pubmed-author:PalmerTrenisT | lld:pubmed |
| pubmed-article:15857612 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15857612 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:15857612 | pubmed:volume | 69 | lld:pubmed |
| pubmed-article:15857612 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15857612 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15857612 | pubmed:pagination | 1483-92 | lld:pubmed |
| pubmed-article:15857612 | pubmed:dateRevised | 2008-9-11 | lld:pubmed |
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| pubmed-article:15857612 | pubmed:meshHeading | pubmed-meshheading:15857612... | lld:pubmed |
| pubmed-article:15857612 | pubmed:meshHeading | pubmed-meshheading:15857612... | lld:pubmed |
| pubmed-article:15857612 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15857612 | pubmed:articleTitle | Ca2+ sensitization and the regulation of contractility in rat anococcygeus and retractor penis muscle. | lld:pubmed |
| pubmed-article:15857612 | pubmed:affiliation | Department of Physiology, Medical College of Georgia, Augusta, GA 30912-3000, USA. cteixeira@mail.mcg.edu <cteixeira@mail.mcg.edu> | lld:pubmed |
| pubmed-article:15857612 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15857612 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:15857612 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:15857612 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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